ALDH4A1 Antibody - #DF7044

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Product: ALDH4A1 Antibody
Catalog: DF7044
Description: Rabbit polyclonal antibody to ALDH4A1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 62kDa; 62kD(Calculated).
Uniprot: P30038
RRID: AB_2839000

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Chicken(83%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
ALDH4A1 Antibody detects endogenous levels of total ALDH4A1.
RRID:
AB_2839000
Cite Format: Affinity Biosciences Cat# DF7044, RRID:AB_2839000.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AL4A1_HUMAN; aldehyde dehydrogenase 4; aldehyde dehydrogenase 4 family, member A1; Aldehyde dehydrogenase family 4 member A1; Aldehyde dehydrogenase, family 4, subfamily A, member 1; ALDH4; aldh4a1; Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial; Delta-1-pyrroline-5-carboxylate dehydrogenase; L-glutamate gamma-semialdehyde dehydrogenase; mitochondrial; mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase; P5C dehydrogenase; P5CD; P5CDh; P5CDhL; P5CDhS; Pyrroline-5-carboxylate dehydrogenase; RP11 128M10.1;

Immunogens

Immunogen:
Uniprot:

P30038(AL4A1_HUMAN) >>Visit HPA database.

Gene(ID):
ALDH4A1(8659)
Expression:
P30038 AL4A1_HUMAN:

Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.

Description:
This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Sequence:
MLLPAPALRRALLSRPWTGAGLRWKHTSSLKVANEPVLAFTQGSPERDALQKALKDLKGRMEAIPCVVGDEEVWTSDVQYQVSPFNHGHKVAKFCYADKSLLNKAIEAALAARKEWDLKPIADRAQIFLKAADMLSGPRRAEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGLEGFVAAISPFNFTAIGGNLAGAPALMGNVVLWKPSDTAMLASYAVYRILREAGLPPNIIQFVPADGPLFGDTVTSSEHLCGINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVSGTLRSAFEYGGQKCSACSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFGTFFSAVIDAKSFARIKKWLEHARSSPSLTILAGGKCDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSVYVYPDDKYKETLQLVDSTTSYGLTGAVFSQDKDVVQEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYAYMQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Xenopus
92
Rabbit
92
Chicken
83
Pig
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30038 As Substrate

Site PTM Type Enzyme
S44 Phosphorylation
K93 Acetylation
Y96 Phosphorylation
K153 Methylation
R304 Methylation
S326 Phosphorylation
S331 Phosphorylation
T336 Phosphorylation
K462 Acetylation
K495 Methylation
K495 Ubiquitination
R498 Methylation
Y505 Phosphorylation
T528 Phosphorylation
K552 Acetylation

Research Backgrounds

Function:

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the aldehyde dehydrogenase family.

Research Fields

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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