SDHA Antibody - #DF7043

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Product: SDHA Antibody
Catalog: DF7043
Description: Rabbit polyclonal antibody to SDHA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 72kDa; 73kD(Calculated).
Uniprot: P31040
RRID: AB_2838999

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SDHA Antibody detects endogenous levels of total SDHA.
RRID:
AB_2838999
Cite Format: Affinity Biosciences Cat# DF7043, RRID:AB_2838999.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CMD1GG; DHSA_HUMAN; Flavoprotein subunit of complex II; Fp; PGL5; SDH 2; SDH1; SDH2; SDHA; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Succinate dehydrogenase complex flavoprotein subunit A; Succinate dehydrogenase complex flavoprotein subunit; Succinate dehydrogenase complex flavoprotein subunit precursor; Succinate dehydrogenase complex subunit A; Succinate dehydrogenase complex subunit A flavoprotein (Fp); Succinate dehydrogenase complex subunit A flavoprotein;

Immunogens

Immunogen:
Uniprot:

P31040(SDHA_HUMAN) >>Visit HPA database.

Gene(ID):
SDHA(6389)
Description:
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.
Sequence:
MSGVRGLSRLLSARRLALAKAWPTVLQTGTRGFHFTVDGNKRASAKVSDSISAQYPVVDHEFDAVVVGAGGAGLRAAFGLSEAGFNTACVTKLFPTRSHTVAAQGGINAALGNMEEDNWRWHFYDTVKGSDWLGDQDAIHYMTEQAPAAVVELENYGMPFSRTEDGKIYQRAFGGQSLKFGKGGQAHRCCCVADRTGHSLLHTLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIEDGSIHRIRAKNTVVATGGYGRTYFSCTSAHTSTGDGTAMITRAGLPCQDLEFVQFHPTGIYGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
83
Chicken
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31040 As Substrate

Site PTM Type Enzyme
T24 Phosphorylation
K41 Ubiquitination
K92 Ubiquitination
K167 Ubiquitination
S177 Phosphorylation
K179 Acetylation
K179 Ubiquitination
Y215 Phosphorylation P12931 (SRC)
S243 Phosphorylation
K250 Ubiquitination
S321 Phosphorylation
K335 Acetylation
K335 Ubiquitination
K361 Acetylation
Y365 Phosphorylation
K418 Ubiquitination
Y436 Phosphorylation
S456 Phosphorylation
K485 Ubiquitination
K498 Acetylation
K498 Ubiquitination
R500 Methylation
R507 Methylation
R512 Methylation
K517 Ubiquitination
S530 Phosphorylation
K538 Ubiquitination
K541 Acetylation
K541 Ubiquitination
Y543 Phosphorylation P09769 (FGR)
K547 Acetylation
K547 Methylation
K547 Ubiquitination
R585 Methylation
R589 Methylation
R594 Methylation
K598 Acetylation
Y604 Phosphorylation P09769 (FGR)
Y606 Phosphorylation
K608 Acetylation
K608 Ubiquitination
K616 Ubiquitination
K624 Acetylation
K624 Ubiquitination
S628 Phosphorylation
Y629 Phosphorylation
K647 Ubiquitination
R662 Methylation

Research Backgrounds

Function:

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.

PTMs:

Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation.

Acetylated. Deacetylated by SIRT3.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD (By similarity). Interacts with SDHAF2/SDH5; interaction is required for FAD attachment. Interacts with TRAP1.

Family&Domains:

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

References

1). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019 (PubMed: 30703481) [IF=7.1]

Application: WB    Species: human    Sample: ACHN cells

Fig. 5. |Desuccinylation of SDHA promotes ccRCC cell proliferation via inhibiting its catalytic activity and weakening SDH5 binding. (A) Empty vector, Flag-tagged WT SDHA or the K547R mutant was ectopically expressed in ACHN cells, succinylation level of SDHA was detected by immunoprecipitating SDHA and blotting with anti-pan-succinylation antibody. In vector transfected control cells with no flag expression, endogenous SDHA was not immunoprecipitated by flag antibody and no succinylation signal was detected. SDHA WT was succinylated and K547R mutant was hardly succinylated.

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