SDHA Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

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SDHA Antibody - Western blot analysis of extracts from Hela, using SDHA Antibody.

SDHA Antibody - Western blot analysis of extracts from P19 cells, using SDHA Antibody.

SDHA Antibody - DF7043 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P.

SDHA Antibody - DF7043 staining Hela cells by IF/ICC.

SDHA Antibody - Fig.

Product:	SDHA Antibody
Catalog:	DF7043
Description:	Rabbit polyclonal antibody to SDHA
Application:	WB IHC IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	72kDa; 73kD(Calculated).
Uniprot:	P31040
RRID:	AB_2838999

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SDHA Antibody detects endogenous levels of total SDHA.

RRID:

AB_2838999
Cite Format: Affinity Biosciences Cat# DF7043, RRID:AB_2838999.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CMD1GG; DHSA_HUMAN; Flavoprotein subunit of complex II; Fp; PGL5; SDH 2; SDH1; SDH2; SDHA; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Succinate dehydrogenase complex flavoprotein subunit A; Succinate dehydrogenase complex flavoprotein subunit; Succinate dehydrogenase complex flavoprotein subunit precursor; Succinate dehydrogenase complex subunit A; Succinate dehydrogenase complex subunit A flavoprotein (Fp); Succinate dehydrogenase complex subunit A flavoprotein;

Immunogens

Immunogen:

A synthesized peptide derived from human SDHA, corresponding to a region within the internal amino acids.

Uniprot:

P31040(SDHA_HUMAN) >>Visit HPA database.

Gene(ID):

SDHA(6389)

Description:

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.

Sequence:

P31040 SDHA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGVRGLSRLLSARRLALAKAWPTVLQTGTRGFHFTVDGNKRASAKVSDSISAQYPVVDHEFDAVVVGAGGAGLRAAFGLSEAGFNTACVTKLFPTRSHTVAAQGGINAALGNMEEDNWRWHFYDTVKGSDWLGDQDAIHYMTEQAPAAVVELENYGMPFSRTEDGKIYQRAFGGQSLKFGKGGQAHRCCCVADRTGHSLLHTLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIEDGSIHRIRAKNTVVATGGYGRTYFSCTSAHTSTGDGTAMITRAGLPCQDLEFVQFHPTGIYGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Horse

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P31040_SDHA_HUMAN

Function:

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.

PTMs:

Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation.

Acetylated. Deacetylated by SIRT3.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Family&Domains:

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

References

1). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019 (PubMed: 30703481) [IF=7.1]

Application: WB Species: human Sample: ACHN cells

Fig. 5. |Desuccinylation of SDHA promotes ccRCC cell proliferation via inhibiting its catalytic activity and weakening SDH5 binding. (A) Empty vector, Flag-tagged WT SDHA or the K547R mutant was ectopically expressed in ACHN cells, succinylation level of SDHA was detected by immunoprecipitating SDHA and blotting with anti-pan-succinylation antibody. In vector transfected control cells with no flag expression, endogenous SDHA was not immunoprecipitated by flag antibody and no succinylation signal was detected. SDHA WT was succinylated and K547R mutant was hardly succinylated.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SDHA Antibody - #DF7043