FES Antibody - #DF7031

(1)  
Product: FES Antibody
Catalog: DF7031
Description: Rabbit polyclonal antibody to FES
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 93kDa; 93kD(Calculated).
Uniprot: P07332
RRID: AB_2838987

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(86%), Horse(86%), Sheep(86%)
Clonality:
Polyclonal
Specificity:
FES Antibody detects endogenous levels of total FES.
RRID:
AB_2838987
Cite Format: Affinity Biosciences Cat# DF7031, RRID:AB_2838987.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

c-fes/fps protein; cFES; Feline sarcoma (Snyder Theilen) viral (v fes)/Fujinami avian sarcoma (PRCII) viral (v fps) oncogene homolog; feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami; Feline sarcoma oncogene; Feline sarcoma/Fujinami avian sarcoma oncogene homolog; fes; FES proto oncogene, tyrosine kinase; FES_HUMAN; FPS; Oncogene FES; Oncogene FES feline sarcoma virus; P93c fes; Proto oncogene tyrosine protein kinase Fes/Fps; Proto-oncogene c-Fes; Proto-oncogene c-Fps; proto-oncogene tyrosine-protein kinase Fes/Fps; Tyrosine-protein kinase Fes/Fps;

Immunogens

Immunogen:
Uniprot:

P07332(FES_HUMAN) >>Visit HPA database.

Gene(ID):
FES(2242)
Expression:
P07332 FES_HUMAN:

Widely expressed. Detected in adult colon epithelium (at protein level) (PubMed:16455651, PubMed:19051325). Expressed in melanocytes (at protein level) (PubMed:28463229).

Description:
This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.
Sequence:
MGFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHMSLQDSGGQSRAISPDSPISQSWAEITSQTEGLSRLLRQHAEDLNSGPLSKLSLLIRERQQLRKTYSEQWQQLQQELTKTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPVLLLQDDRHSTSSSEQEREGGRTPTLEILKSHISGIFRPKFSLPPPLQLIPEVQKPLHEQLWYHGAIPRAEVAELLVHSGDFLVRESQGKQEYVLSVLWDGLPRHFIIQSLDNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHRAVPKDKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLPPDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQPIYIVMELVQGGDFLTFLRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVLKISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFGILLWETFSLGASPYPNLSNQQTREFVEKGGRLPCPELCPDAVFRLMEQCWAYEPGQRPSFSTIYQELQSIRKRHR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
86
Bovine
86
Sheep
86
Zebrafish
71
Chicken
71
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07332 As Substrate

Site PTM Type Enzyme
S64 Phosphorylation
S67 Phosphorylation
S100 Phosphorylation
S149 Phosphorylation
Y156 Phosphorylation
Y261 Phosphorylation
S373 Phosphorylation
S408 Phosphorylation
T409 Phosphorylation
S410 Phosphorylation
S411 Phosphorylation
S412 Phosphorylation
T421 Phosphorylation
T423 Phosphorylation
S432 Phosphorylation
Y461 Phosphorylation
S477 Phosphorylation
T595 Phosphorylation
Y614 Phosphorylation
K698 Ubiquitination
Y713 Phosphorylation P07332 (FES)
S716 Phosphorylation
K725 Ubiquitination
Y734 Phosphorylation
K775 Ubiquitination
Y811 Phosphorylation P07332 (FES)

PTMs - P07332 As Enzyme

Substrate Site Source
P07332 (FES) Y713 Uniprot
P07332 (FES) Y811 Uniprot
P11274 (BCR) Y177 Uniprot
P11274-1 (BCR) Y246 Uniprot
P11274 (BCR) Y276 Uniprot
P11274 (BCR) Y279 Uniprot
P11274 (BCR) Y283 Uniprot
P46459 (NSF) Y83 Uniprot

Research Backgrounds

Function:

Tyrosine-protein kinase that acts downstream of cell surface receptors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, cell attachment and cell spreading. Plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Acts down-stream of the activated FCER1 receptor and the mast/stem cell growth factor receptor KIT. Plays a role in the regulation of mast cell degranulation. Plays a role in the regulation of cell differentiation and promotes neurite outgrowth in response to NGF signaling. Plays a role in cell scattering and cell migration in response to HGF-induced activation of EZR. Phosphorylates BCR and down-regulates BCR kinase activity. Phosphorylates HCLS1/HS1, PECAM1, STAT3 and TRIM28.

PTMs:

Autophosphorylated on Tyr-713. Phosphorylated by LYN in response to FCER1 activation. Phosphorylated by HCK.

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle. Golgi apparatus. Cell junction>Focal adhesion.
Note: Distributed throughout the cytosol when the kinase is not activated. Association with microtubules requires activation of the kinase activity. Shuttles between focal adhesions and cell-cell contacts in epithelial cells. Recruited to the lateral cell membrane in polarized epithelial cells by interaction with phosphorylated EZR. Detected at tubular membrane structures in the cytoplasm and at the cell periphery.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Detected in adult colon epithelium (at protein level). Expressed in melanocytes (at protein level).

Subunit Structure:

Homooligomer. Interacts with BCR. Interacts (when activated, via coiled coil domain) with TRIM28. Interacts (via SH2 domain) with phosphorylated EZR, MS4A2/FCER1B and HCLS1/HS1. Interacts with phosphorylated KIT. Interacts with FLT3. Interacts (via F-BAR domain) with soluble tubulin. Interacts (via SH2 domain) with microtubules.

Family&Domains:

The coiled coil domains are important for regulating the kinase activity. They mediate homooligomerization and probably also interaction with other proteins.

The N-terminal region including the first coiled coil domain mediates interaction with phosphoinositide-containing membranes.

Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.