Cytokeratin 7 Antibody - #DF7027

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Product: Cytokeratin 7 Antibody
Catalog: DF7027
Description: Rabbit polyclonal antibody to Cytokeratin 7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: P08729
RRID: AB_2838983

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Sheep(88%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 7 Antibody detects endogenous levels of total Cytokeratin 7.
RRID:
AB_2838983
Cite Format: Affinity Biosciences Cat# DF7027, RRID:AB_2838983.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 7; CK-7; CK7; Cytokeratin 7; Cytokeratin-7; D15Wsu77e; K2C7; K2C7_HUMAN; K7; Keratin 7; Keratin 7, type II; Keratin type II cytoskeletal 7; Keratin, 55K type II cytoskeletal; Keratin, simple epithelial; Keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 7; Keratin-7; Krt2-7; KRT7; MGC11625; MGC129731; MGC3625; Sarcolectin; SCL; Type II mesothelial keratin K7; Type-II keratin Kb7;

Immunogens

Immunogen:
Uniprot:

P08729(K2C7_HUMAN) >>Visit HPA database.

Gene(ID):
KRT7(3855)
Expression:
P08729 K2C7_HUMAN:

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

Description:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Sequence:
MSIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVAVRSAYGGPVGAGIREVTINQSLLAPLRLDADPSLQRVRQEESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINHRTAAENEFVVLKKDVDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRLAGDGVGAVNISVMNSTGGSSSGGGIGLTLGGTMGSNALSFSSSAGPGLLKAYSIRTASASRRSARD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
88
Sheep
88
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08729 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S6 Phosphorylation
S7 Phosphorylation
S14 Phosphorylation
S18 Phosphorylation
R20 Methylation
R25 Methylation
S27 Phosphorylation
S28 Phosphorylation
S36 Phosphorylation
S37 Phosphorylation
S38 Phosphorylation
Y40 Phosphorylation
S45 Phosphorylation
R46 Methylation
R48 Methylation
S53 Phosphorylation
Y55 Phosphorylation
T67 Phosphorylation
S71 Phosphorylation
S83 Phosphorylation
K96 Ubiquitination
T97 Phosphorylation
K101 Acetylation
K101 Methylation
K101 Sumoylation
K101 Ubiquitination
S104 Phosphorylation
K108 Acetylation
K108 Ubiquitination
K117 Ubiquitination
K122 Ubiquitination
K130 Sumoylation
K130 Ubiquitination
S134 Phosphorylation
S168 Phosphorylation
K179 Acetylation
Y180 Phosphorylation
K198 Ubiquitination
K199 Acetylation
K199 Ubiquitination
Y205 Phosphorylation
S207 Phosphorylation
K214 Ubiquitination
T227 Phosphorylation
T231 Phosphorylation
T234 Phosphorylation
S238 Phosphorylation
S241 Phosphorylation
T243 Phosphorylation
S244 Phosphorylation
S248 Phosphorylation
S252 Phosphorylation
S254 Phosphorylation
K265 Ubiquitination
Y268 Phosphorylation
K273 Ubiquitination
S275 Phosphorylation
Y283 Phosphorylation
K286 Ubiquitination
T289 Phosphorylation
K296 Acetylation
K296 Ubiquitination
K326 Sumoylation
K326 Ubiquitination
K331 Ubiquitination
K348 Ubiquitination
K353 Ubiquitination
Y375 Phosphorylation
S380 Phosphorylation
K394 Acetylation
K394 Sumoylation
K394 Ubiquitination
S401 Phosphorylation
S456 Phosphorylation
T459 Phosphorylation
S461 Phosphorylation
S463 Phosphorylation
S466 Phosphorylation

Research Backgrounds

Function:

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

PTMs:

Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10. Interacts with GPER1.

(Microbial infection) Interacts with human papillomavirus 16/HPV16 protein E7.

Family&Domains:

Belongs to the intermediate filament family.

References

1). Isolation, culture, and identification of ceruminous gland cells. Journal of Molecular Histology, 2022 (PubMed: 35113280) [IF=2.9]
2). Establishment and Characterization of a New Cell Line from Enzootic Nasal Adenocarcinoma in Goats: ENA-1. Veterinary sciences, 2024 (PubMed: 38922007) [IF=2.4]

Application: IF/ICC    Species: Mouse    Sample: ENA-1 cells

Figure 5 Immunofluorescence staining of ENA-1 cells for CK7 and CK18. ENA-1 cells expressed CK7 and CK18 in the cytoplasm, with CK18 most strongly expressed. (A) Negative control; (B) ENA-1 cells expressing CK7; and (C) ENA-1 cells strongly expressing CK18. (A–C), bar—100 μm.
3). Bronchioloalveolar carcinoma not related to jaagsiekte sheep retrovirus in a goat. Veterinary pathology, 2007 (PubMed: 17846248) [IF=2.3]

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