PCBP2 Antibody - #DF6991

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Product: PCBP2 Antibody
Catalog: DF6991
Description: Rabbit polyclonal antibody to PCBP2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: Q15366
RRID: AB_2838947

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%)
Clonality:
Polyclonal
Specificity:
PCBP2 Antibody detects endogenous levels of total PCBP2.
RRID:
AB_2838947
Cite Format: Affinity Biosciences Cat# DF6991, RRID:AB_2838947.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha CP2; Alpha-CP2; alphaCP-2; Cbp; CTBP; Heterogeneous nuclear ribonucleoprotein E2; Heterogenous nuclear ribonucleoprotein E2; hnRNP E2; hnRNP-E2; HNRNPE2; Hnrnpx; HNRPE2; Hnrpx; MGC110998; PCBP2; PCBP2_HUMAN; poly(rC) binding protein 2; Poly(rC)-binding protein 2; Putative heterogeneous nuclear ribonucleoprotein X; rCbinding protein 2;

Immunogens

Immunogen:
Uniprot:

Q15366(PCBP2_HUMAN) >>Visit HPA database.

Gene(ID):
PCBP2(5094)
Expression:
Q15366 PCBP2_HUMAN:

Detected in all tissues examined.

Description:
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Sequence:
MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESGARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLEEDISSSMTNSTAASRPPVTLRLVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVKQICVVMLETLSQSPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGSDSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQPDLTKLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWGLDASAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVEGSTDRQVTITGSAASISLAQYLINVRLSSETGGMGSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15366 As Substrate

Site PTM Type Enzyme
T15 Phosphorylation
K23 Acetylation
K23 Sumoylation
K23 Ubiquitination
S27 Phosphorylation
K31 Acetylation
K31 Sumoylation
K31 Ubiquitination
S50 Phosphorylation
C54 S-Nitrosylation
K70 Acetylation
T99 Phosphorylation
S107 Phosphorylation
C109 S-Nitrosylation
S111 Phosphorylation
K115 Sumoylation
K115 Ubiquitination
K119 Ubiquitination
S154 Phosphorylation
C158 S-Nitrosylation
K160 Ubiquitination
S173 Phosphorylation P11274 (BCR) , P27361 (MAPK3) , P28482 (MAPK1)
T179 Phosphorylation
Y182 Phosphorylation
K185 Acetylation
K185 Ubiquitination
S187 Phosphorylation
S188 Phosphorylation
S189 Phosphorylation P11274 (BCR) , P27361 (MAPK3) , P28482 (MAPK1)
T202 Phosphorylation
T213 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , P11274 (BCR)
Y230 Phosphorylation
T231 Phosphorylation
Y236 Phosphorylation
K245 Ubiquitination
S266 Phosphorylation
S270 Phosphorylation
S271 Phosphorylation
S272 Phosphorylation P11274 (BCR) , P27361 (MAPK3) , P28482 (MAPK1)
K276 Sumoylation
Y278 Phosphorylation
S289 Phosphorylation
K309 Acetylation
K309 Sumoylation
K309 Ubiquitination
R314 Methylation
K322 Sumoylation
K322 Ubiquitination
S340 Phosphorylation
S364 Phosphorylation
S365 Phosphorylation

Research Backgrounds

Function:

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation.

(Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation. Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.

PTMs:

Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.

(Microbial infection) Proteolyticaly cleaved by picornavirus proteinase 3CD.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in all tissues examined.

Subunit Structure:

Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with IFIH1 and RNF135. Interacts with MAVS (via C-terminus) and ITCH (via WW domains).

Family&Domains:

The KH domains mediates poly(C) binding.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

References

1). d-Borneol enhances cisplatin sensitivity via autophagy dependent EMT signaling and NCOA4-mediated ferritinophagy. PHYTOMEDICINE, 2022 (PubMed: 36030746) [IF=7.9]

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