HNMT Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A2780 cells, using HNMT Antibody.

Product:	HNMT Antibody
Catalog:	DF6982
Description:	Rabbit polyclonal antibody to HNMT
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	33kDa; 33kD(Calculated).
Uniprot:	P50135
RRID:	AB_2838938

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

HNMT Antibody detects endogenous levels of total HNMT.

RRID:

AB_2838938
Cite Format: Affinity Biosciences Cat# DF6982, RRID:AB_2838938.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC 2.1.1.8; Histamine N methyltransferase; Histamine N-methyltransferase; HMT; HNMT; HNMT S1; HNMT S2; HNMT_HUMAN;

Immunogens

Immunogen:

Uniprot:

P50135(HNMT_HUMAN) >>Visit HPA database.

Gene(ID):

HNMT(3176)

Description:

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Sequence:

P50135 HNMT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50135 As Substrate

P50135

Site	PTM Type	Source
S10	Phosphorylation	Uniprot
S52	Phosphorylation	Uniprot
K99	Ubiquitination	Uniprot
K104	Ubiquitination	Uniprot
K130	Ubiquitination	Uniprot
K131	Ubiquitination	Uniprot
T164	Phosphorylation	Uniprot
S174	Phosphorylation	Uniprot
S176	Phosphorylation	Uniprot
S177	Phosphorylation	Uniprot
T284	Phosphorylation	Uniprot

Research Backgrounds

P50135_HNMT_HUMAN

Function:

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. HNMT family.

Research Fields

· Metabolism > Amino acid metabolism > Histidine metabolism.

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HNMT Antibody - #DF6982