TCOF1 Antibody - #DF6977

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Product: TCOF1 Antibody
Catalog: DF6977
Description: Rabbit polyclonal antibody to TCOF1
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 152kDa; 152kD(Calculated).
Uniprot: Q13428
RRID: AB_2838933

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
TCOF1 Antibody detects endogenous levels of total TCOF1.
RRID:
AB_2838933
Cite Format: Affinity Biosciences Cat# DF6977, RRID:AB_2838933.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Mandibulofacial dysostosis; MFD1; Nucleolar trafficking phosphoprotein; TCOF 1; TCOF_HUMAN; TCOF1; TCS; TCS1; Treacher Collins Franceschetti syndrome 1; Treacher Collins syndrome; Treacher Collins syndrome protein; Treacle; Treacle protein;

Immunogens

Immunogen:
Uniprot:

Q13428(TCOF_HUMAN) >>Visit HPA database.

Gene(ID):
TCOF1(6949)
Description:
This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene.
Sequence:
MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
88
Rabbit
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13428 As Substrate

Site PTM Type Enzyme
Y15 Phosphorylation
K74 Acetylation
S83 Phosphorylation
T84 Phosphorylation
S85 Phosphorylation
S87 Phosphorylation
S88 Phosphorylation
T102 Phosphorylation
S107 Phosphorylation
T108 Phosphorylation
S110 Phosphorylation
S111 Phosphorylation
S119 Phosphorylation
K122 Acetylation
K122 Ubiquitination
K124 Acetylation
K124 Methylation
K126 Acetylation
S138 Phosphorylation
T144 Phosphorylation
K146 Acetylation
T147 Phosphorylation
S153 Phosphorylation
K155 Acetylation
S156 Phosphorylation P06493 (CDK1)
S160 Phosphorylation
S164 Phosphorylation
T168 Phosphorylation
S171 Phosphorylation
T173 Phosphorylation
S178 Phosphorylation
T210 Phosphorylation
S227 Phosphorylation
S229 Phosphorylation
T230 Phosphorylation
S233 Phosphorylation
K244 Acetylation
T249 Phosphorylation
S270 Phosphorylation
S272 Phosphorylation
S273 Phosphorylation
S277 Phosphorylation
S279 Phosphorylation
K296 Acetylation
S304 Phosphorylation
T310 Phosphorylation
T316 Phosphorylation
K322 Acetylation
S328 Phosphorylation
S342 Phosphorylation
S343 Phosphorylation
S346 Phosphorylation
S347 Phosphorylation
S349 Phosphorylation
S365 Phosphorylation
T368 Phosphorylation
S369 Phosphorylation
S375 Phosphorylation
S381 Phosphorylation
K393 Acetylation
T394 Phosphorylation
S410 Phosphorylation
S412 Phosphorylation
S413 Phosphorylation
S414 Phosphorylation
S417 Phosphorylation
S419 Phosphorylation
S440 Phosphorylation
S446 Phosphorylation
K449 Methylation
S475 Phosphorylation
S477 Phosphorylation
S478 Phosphorylation
S479 Phosphorylation
S482 Phosphorylation
S484 Phosphorylation
S503 Phosphorylation
K507 Acetylation
T511 Phosphorylation
T533 Phosphorylation
S549 Phosphorylation
S555 Phosphorylation
S556 Phosphorylation
S571 Phosphorylation
K579 Acetylation
T581 Phosphorylation
S582 Phosphorylation P24941 (CDK2)
S583 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
K591 Ubiquitination
K600 Acetylation
K600 Sumoylation
S608 Phosphorylation
S610 Phosphorylation
S611 Phosphorylation
S614 Phosphorylation
S615 Phosphorylation
S617 Phosphorylation
S620 Phosphorylation
T663 Phosphorylation
S674 Phosphorylation
S679 Phosphorylation
S693 Phosphorylation
S699 Phosphorylation
S701 Phosphorylation
S728 Phosphorylation
K732 Acetylation
K732 Methylation
K732 Sumoylation
S734 Phosphorylation
K746 Acetylation
K755 Acetylation
K755 Sumoylation
S762 Phosphorylation
S764 Phosphorylation
S765 Phosphorylation
S769 Phosphorylation
S771 Phosphorylation
S777 Phosphorylation
S784 Phosphorylation
S801 Phosphorylation
T805 Phosphorylation
K811 Acetylation
T814 Phosphorylation
S823 Phosphorylation
S825 Phosphorylation
T838 Phosphorylation
S846 Phosphorylation
S849 Phosphorylation
S868 Phosphorylation
S870 Phosphorylation
S871 Phosphorylation
S875 Phosphorylation
S877 Phosphorylation
S890 Phosphorylation
K904 Acetylation
S906 Phosphorylation
T914 Phosphorylation
S932 Phosphorylation
S934 Phosphorylation
S935 Phosphorylation
S936 Phosphorylation
S939 Phosphorylation
S941 Phosphorylation
S967 Phosphorylation
S982 Phosphorylation P24941 (CDK2)
T983 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
S990 Phosphorylation
S992 Phosphorylation
T993 Phosphorylation
S996 Phosphorylation
S997 Phosphorylation
S998 Phosphorylation
S999 Phosphorylation
S1001 Phosphorylation
C1012 S-Nitrosylation
T1026 Phosphorylation
S1036 Phosphorylation
S1041 Phosphorylation
S1047 Phosphorylation
T1060 Phosphorylation
S1069 Phosphorylation
T1073 Phosphorylation
K1078 Acetylation
T1094 Phosphorylation
T1110 Phosphorylation
S1111 Phosphorylation
S1114 Phosphorylation
T1115 Phosphorylation
S1116 Phosphorylation
S1142 Phosphorylation
S1143 Phosphorylation
S1168 Phosphorylation
T1171 Phosphorylation
T1175 Phosphorylation
T1179 Phosphorylation
T1181 Phosphorylation
T1186 Phosphorylation
S1190 Phosphorylation
S1191 Phosphorylation
S1199 Phosphorylation
S1204 Phosphorylation
Y1206 Phosphorylation
T1208 Phosphorylation
T1222 Phosphorylation
S1227 Phosphorylation
S1228 Phosphorylation
S1230 Phosphorylation
S1232 Phosphorylation
S1233 Phosphorylation
T1234 Phosphorylation
S1257 Phosphorylation
T1260 Phosphorylation
K1263 Acetylation
T1269 Phosphorylation
T1270 Phosphorylation
S1287 Phosphorylation
T1288 Phosphorylation
T1296 Phosphorylation
K1335 Ubiquitination
K1346 Acetylation
K1346 Ubiquitination
S1350 Phosphorylation
T1358 Phosphorylation
S1361 Phosphorylation
K1362 Acetylation
S1376 Phosphorylation
S1378 Phosphorylation
T1382 Phosphorylation
T1385 Phosphorylation
S1386 Phosphorylation
K1403 Acetylation
K1405 Acetylation
S1407 Phosphorylation
S1410 Phosphorylation
K1414 Acetylation
K1414 Sumoylation
S1434 Phosphorylation
S1471 Phosphorylation
S1473 Phosphorylation

Research Backgrounds

Function:

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.

PTMs:

Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer; heterodimerizes with NOLC1 following monoubiquitination. Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with NOP56 in an RNA-independent manner.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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