EEF1D Antibody - #DF6974

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Product: EEF1D Antibody
Catalog: DF6974
Description: Rabbit polyclonal antibody to EEF1D
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 31kDa,37kDa; 31kD(Calculated).
Uniprot: P29692
RRID: AB_2838930

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
EEF1D Antibody detects endogenous levels of total EEF1D.
RRID:
AB_2838930
Cite Format: Affinity Biosciences Cat# DF6974, RRID:AB_2838930.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EEF1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); Eukaryotic translation elongation factor 1 delta; FP1047; Guanine nucleotide exchange protein;

Immunogens

Immunogen:
Uniprot:

P29692(EF1D_HUMAN) >>Visit HPA database.

Gene(ID):
EEF1D(1936)
Expression:
P29692 EF1D_HUMAN:

Isoform 2 is specifically expressed in brain, cerebellum and testis.

Description:
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
Sequence:
MATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29692 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K10 Acetylation
K10 Ubiquitination
K15 Acetylation
K15 Ubiquitination
K17 Acetylation
K17 Ubiquitination
Y18 Phosphorylation
R24 Methylation
Y26 Phosphorylation
S37 Phosphorylation
S44 Phosphorylation
K59 Ubiquitination
S60 Phosphorylation
S64 Phosphorylation
S65 Phosphorylation
S70 Phosphorylation
T73 Phosphorylation
S74 Phosphorylation
S86 Phosphorylation
S93 Phosphorylation
R95 Methylation
S106 Phosphorylation
K107 Acetylation
K107 Ubiquitination
K117 Acetylation
K117 Ubiquitination
S118 Phosphorylation
S119 Phosphorylation
T125 Phosphorylation
T129 Phosphorylation
S133 Phosphorylation P06493 (CDK1)
T147 Phosphorylation
S162 Phosphorylation P68400 (CSNK2A1)
K169 Ubiquitination
R178 Methylation
Y182 Phosphorylation
K185 Ubiquitination
K186 Ubiquitination
K188 Ubiquitination
K189 Ubiquitination
S197 Phosphorylation
K203 Acetylation
K203 Sumoylation
S220 Phosphorylation
S231 Phosphorylation
K232 Ubiquitination
Y238 Phosphorylation
K242 Ubiquitination
K265 Ubiquitination
K280 Ubiquitination

Research Backgrounds

Function:

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.

Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 2 is specifically expressed in brain, cerebellum and testis.

Subunit Structure:

EF-1 is composed of 4 subunits: alpha, beta, delta isoform 1, and gamma. Isoform 2 interacts with HSF1 and NFE2L2.

Family&Domains:

Belongs to the EF-1-beta/EF-1-delta family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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