PSMC4 Antibody - #DF6973

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Product: PSMC4 Antibody
Catalog: DF6973
Description: Rabbit polyclonal antibody to PSMC4
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 47kDa; 47kD(Calculated).
Uniprot: P43686
RRID: AB_2838929

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PSMC4 Antibody detects endogenous levels of total PSMC4.
RRID:
AB_2838929
Cite Format: Affinity Biosciences Cat# DF6973, RRID:AB_2838929.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

26S protease regulatory subunit 6B; 26S proteasome AAA ATPase subunit RPT3; 26S proteasome AAA-ATPase subunit RPT3; MB67 interacting protein; MB67-interacting protein; MIP224; Protease 26S subunit 6; Proteasome (prosome macropain) 26S subunit ATPase 4; Proteasome 19S S6; Proteasome 26S subunit ATPase 4; Proteasome 26S subunit, ATPase, 4; PRS6B_HUMAN; PSMC4; RPT3; S6; Tat binding protein 7; TAT-binding protein 7; TBP 7; TBP-7;

Immunogens

Immunogen:

A synthesized peptide derived from human PSMC4, corresponding to a region within C-terminal amino acids.

Uniprot:

P43686(PRS6B_HUMAN) >>Visit HPA database.

Gene(ID):
PSMC4(5704)
Description:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Sequence:
MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC4 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the AAA ATPase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

References

1). L-leucine dietary supplementation modulates muscle protein degradation and increases pro-inflammatory cytokines in tumour-bearing rats. CYTOKINE, 2017 (PubMed: 28494385) [IF=3.7]

Application: WB    Species: rat    Sample: gastrocnemius muscle tissue

Fig. 3.| Muscle mass-to-body weight ratio (%; A), total muscle protein concentrations (µg/µL; B) and proteasome subunits: 11S (% of arbitrary unity; C), 19S (% of arbitrary unity; D), and 20Sα (subunits 29 kDa and 32 kDa; (% of arbitrary unity; E)) in the gastrocnemius muscle tissue of Wistar rats with or without tumour and leucine nutritional supplementation. Data are plotted as the mean ± SD. Legend: C: Rats fed a normal protein diet; L: Rats fed a leucine-rich diet; W:Rats fed a normal protein diet and inoculated with Walker 256 tumour cells; WL:Rats fed a leucine-rich diet and inoculated with Walker 256 tumour cells. Samples were collected on the 7th, 14th and 21st days after the beginning of the experiment. **Significant differences as a result of tumour effects, two-way ANOVA with Bonferroni testing, n = 6 per group; *Significant difference as a result of leucine modulation of tumour effects, two-way ANOVA with Bonferroni testing, n = 6 per group. P ≤ 0.05.

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