NUP62 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells, using NUP62 Antibody.

DF6968 at 1/100 staining Human liver tissue by IHC-P.

Product:	NUP62 Antibody
Catalog:	DF6968
Description:	Rabbit polyclonal antibody to NUP62
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	53kDa; 53kD(Calculated).
Uniprot:	P37198
RRID:	AB_2838924

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Sheep(100%), Dog(100%), Chicken(92%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

NUP62 Antibody detects endogenous levels of total NUP62.

RRID:

AB_2838924
Cite Format: Affinity Biosciences Cat# DF6968, RRID:AB_2838924.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; IBSN; MGC841; Nuclear pore glycoprotein p62; nucleoporin 62kDa; Nucleoporin Nup62; nucleoporin p62; nucleoporin p62KD; NUP62; NUP62 protein; p62; SNDI;

Immunogens

Immunogen:

Uniprot:

P37198(NUP62_HUMAN) >>Visit HPA database.

Gene(ID):

NUP62(23636)

Description:

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.

Sequence:

P37198 NUP62_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGFNFGGTGAPTGGFTFGTAKTATTTPATGFSFSTSGTGGFNFGAPFQPATSTPSTGLFSLATQTPATQTTGFTFGTATLASGGTGFSLGIGASKLNLSNTAATPAMANPSGFGLGSSNLTNAISSTVTSSQGTAPTGFVFGPSTTSVAPATTSGGFSFTGGSTAQPSGFNIGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPTPTATITSTGPSLFASIATAPTSSATTGLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATATTTSSSSTTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLESLINKWSLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEELVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Xenopus

Chicken

Horse

Bovine

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P37198 As Substrate

P37198

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
T20	Phosphorylation	Uniprot
T269	Phosphorylation	Uniprot
S272	Phosphorylation	Uniprot
T281	Phosphorylation	Uniprot
K371	Ubiquitination	Uniprot
S408	Phosphorylation	Uniprot
K415	Ubiquitination	Uniprot
S418	Phosphorylation	Uniprot
Y422	Phosphorylation	Uniprot
Y434	Phosphorylation	Uniprot
K435	Ubiquitination	Uniprot
K445	Ubiquitination	Uniprot
K452	Ubiquitination	Uniprot
S468	Phosphorylation	Uniprot
K476	Ubiquitination	Uniprot
K498	Ubiquitination	Uniprot
K504	Ubiquitination	Uniprot
S516	Phosphorylation	Uniprot
T520	Phosphorylation	Uniprot

Research Backgrounds

P37198_NUP62_HUMAN

Function:

Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown.

PTMs:

O-glycosylated. Contains about 10 N-acetylglucosamine side chain sites predicted for the entire protein, among which only one in the C-terminal.

The inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC.

Subcellular Location:

Nucleus>Nuclear pore complex. Cytoplasm>Cytoskeleton>Spindle pole. Nucleus envelope. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Central region of the nuclear pore, within the transporter (PubMed:1915414). During mitotic cell division, it associates with the poles of the mitotic spindle (PubMed:24107630).

Subunit Structure:

Component of the p62 complex, a complex at least composed of NUP62, NUP54, and NUP58 (By similarity). Interacts with NUP88. Interacts with NUTF2 (By similarity). Interacts with HIKESHI. Interacts with OSBPL8. Interacts with CAPG. Interacts with SAS6 and TUBG1 at the centrosome. Interacts with MCM3AP isoform GANP.

(Microbial infection) Interacts with Epstein-barr virus BGLF4; this interaction allows BGLF4 nuclear entry.

Family&Domains:

Contains FG repeats.

Belongs to the nucleoporin NSP1/NUP62 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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NUP62 Antibody - #DF6968