ILF3 Antibody - #DF6965

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Product: ILF3 Antibody
Catalog: DF6965
Description: Rabbit polyclonal antibody to ILF3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 95kDa; 95kD(Calculated).
Uniprot: Q12906
RRID: AB_2838921

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ILF3 Antibody detects endogenous levels of total ILF3.
RRID:
AB_2838921
Cite Format: Affinity Biosciences Cat# DF6965, RRID:AB_2838921.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CBTF; Double stranded RNA binding protein 76; Double-stranded RNA-binding protein 76; DRBF; DRBP76; ILF3; ILF3_HUMAN; Interleukin enhancer binding factor 3; Interleukin enhancer-binding factor 3; M phase phosphoprotein 4; M-phase phosphoprotein 4; MPHOSPH4; MPP4; NF AT 90; NF-AT-90; NF110; NF90; NFAR; Nuclear factor associated with dsRNA; Nuclear factor of activated T cells 90 kDa; Nuclear factor of activated T-cells 90 kDa; TCP80; Translational control protein 80;

Immunogens

Immunogen:
Uniprot:

Q12906(ILF3_HUMAN) >>Visit HPA database.

Gene(ID):
ILF3(3609)
Expression:
Q12906 ILF3_HUMAN:

Ubiquitous.

Description:
This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]
Sequence:
MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLASGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNENPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVVAPAPVVEAVSTPSAAFPSDATAEQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANKKKRAPVPVRGGPKFAAKPHNPGFGMGGPMHNEVPPPPNLRGRGRGGSIRGRGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSQFYSNGGHSGNASGGGGGGGGGSSGYGSYYQGDNYNSPVPPKHAGKKQPHGGQQKPSYGSGYQSHQGQQQSYNQSPYSNYGPPQGKQKGYNHGQGSYSYSNSYNSPGGGGGSDYNYESKFNYSGSGGRSGGNSYGSGGASYNPGSHGGYGGGSGGGSSYQGKQGGYSQSNYNSPGSGQNYSGPPSSYQSSQGGYGRNADHSMNYQYR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Horse
92
Rabbit
92
Xenopus
73
Zebrafish
58
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12906 As Substrate

Site PTM Type Enzyme
R5 Methylation
R12 Methylation
K17 Ubiquitination
Y22 Phosphorylation
S46 Phosphorylation
S57 Phosphorylation
S62 Phosphorylation
S73 Phosphorylation
K81 Acetylation
K100 Acetylation
K100 Ubiquitination
K105 Ubiquitination
K119 Ubiquitination
K127 Ubiquitination
K158 Ubiquitination
S173 Phosphorylation
T188 Phosphorylation
S190 Phosphorylation
K202 Ubiquitination
S209 Phosphorylation
K214 Acetylation
K214 Ubiquitination
K257 Ubiquitination
S258 Phosphorylation
T261 Phosphorylation
R273 Methylation
K297 Ubiquitination
T300 Phosphorylation
T315 Phosphorylation
R323 Methylation
K332 Ubiquitination
S341 Phosphorylation
K342 Ubiquitination
K348 Sumoylation
T356 Phosphorylation
S362 Phosphorylation
T363 Phosphorylation P24941 (CDK2)
T364 Phosphorylation P24941 (CDK2)
Y365 Phosphorylation
T368 Phosphorylation P24941 (CDK2)
S382 Phosphorylation P24941 (CDK2)
S384 Phosphorylation P24941 (CDK2)
K413 Acetylation
K413 Ubiquitination
K419 Methylation
K454 Ubiquitination
K460 Acetylation
T469 Phosphorylation
S476 Phosphorylation
S477 Phosphorylation
S482 Phosphorylation
T486 Phosphorylation
S503 Phosphorylation
T504 Phosphorylation
S506 Phosphorylation
T522 Phosphorylation
K523 Ubiquitination
K540 Ubiquitination
S545 Phosphorylation
T547 Phosphorylation
K553 Ubiquitination
S571 Phosphorylation
K577 Ubiquitination
Y579 Phosphorylation
K587 Acetylation
K587 Ubiquitination
T592 Phosphorylation
K600 Acetylation
K600 Ubiquitination
K601 Acetylation
R609 Methylation
K613 Methylation
R640 Methylation
R642 Methylation
S647 Phosphorylation P31749 (AKT1) , P05771 (PRKCB)
Y665 Phosphorylation
Y672 Phosphorylation
S674 Phosphorylation
K734 Acetylation
S762 Phosphorylation
Y764 Phosphorylation
S765 Phosphorylation
Y767 Phosphorylation
Y777 Phosphorylation
Y784 Phosphorylation
S785 Phosphorylation
Y786 Phosphorylation
S787 Phosphorylation
S789 Phosphorylation
Y790 Phosphorylation
S792 Phosphorylation
S799 Phosphorylation
Y801 Phosphorylation
Y803 Phosphorylation
Y809 Phosphorylation
S810 Phosphorylation
S812 Phosphorylation
R815 Methylation
S816 Phosphorylation
S820 Phosphorylation
Y821 Phosphorylation
S823 Phosphorylation
S827 Phosphorylation
Y828 Phosphorylation
S832 Phosphorylation
Y836 Phosphorylation
S840 Phosphorylation
S844 Phosphorylation
S845 Phosphorylation
Y846 Phosphorylation
Y853 Phosphorylation
Y858 Phosphorylation
S860 Phosphorylation
S863 Phosphorylation
Y867 Phosphorylation
Y874 Phosphorylation
S876 Phosphorylation
S877 Phosphorylation
R883 Methylation
S888 Phosphorylation
Y891 Phosphorylation
Y893 Phosphorylation
R894 Methylation

Research Backgrounds

Function:

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs. As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response. Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs.

PTMs:

Phosphorylated at Thr-188 and Thr-315 by PKR in response to certain RNA viruses. This phosphorylation results in the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3 where it can bind to viral RNAs and impede viral replication.

Methylated by protein arginine N-methyltransferase 1.

Subcellular Location:

Nucleus>Nucleolus. Cytoplasm. Nucleus.
Note: Localizes in the cytoplasm in response to viral infection. The unphosphorylated form is retained in the nucleus by ILF2. Phosphorylation at Thr-188 and Thr-315 causes the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with FUS and SMN. Interacts (via C-terminus) with PRMT1. Forms a complex with ILF2. Can also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of XRCC6/KU70 and XRCC5/KU80. Forms a heteromeric complex with ZNF346 and ILF3. Found in a nuclear export complex with XPO5, ILF3, Ran and double-stranded RNA or double-stranded minihelix VA1 RNA. Found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 and double-stranded RNA. Interacts with XPO5 and ZNF346. Forms a complex with ILF2, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA. Interacts with AGO1 and AGO2. Interacts with DHX36; this interaction occurs in a RNA-dependent manner. Interacts with ELAVL1; this interaction occurs in a RNA-dependent manner.

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