Choline Acetyltransferase Antibody | Affinity Biosciences

WB
IHC
Cites

1/9

Western blot analysis of extracts from 3T3, using CHAT Antibody.

DF6964 at 1/100 staining human Placenta tissue by IHC-P.

DF6964 at 1/100 staining Mouse brain tissue by IHC-P.

DF6964 at 1/100 staining Mouse muscle tissue by IHC-P.

DF6964 at 1/100 staining Rat brain tissue by IHC-P.

DF6964 at 1/100 staining Rat muscle tissue by IHC-P.

Figure 5.

Figure 4: (a) Expression of ChAT at the SCI lesion in the six groups as determined by immunohistochemical staining (×200 original magnification).

Product:	Choline Acetyltransferase Antibody
Catalog:	DF6964
Description:	Rabbit polyclonal antibody to Choline Acetyltransferase
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	82kDa; 83kD(Calculated).
Uniprot:	P28329
RRID:	AB_2838920

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Choline Acetyltransferase Antibody detects endogenous levels of total Choline Acetyltransferase.

RRID:

AB_2838920
Cite Format: Affinity Biosciences Cat# DF6964, RRID:AB_2838920.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; Choline O acetyltransferase; Choline O-acetyltransferase; CLAT_HUMAN; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584;

Immunogens

Immunogen:

A synthesized peptide derived from human Choline Acetyltransferase, corresponding to a region within C-terminal amino acids.

Uniprot:

P28329(CLAT_HUMAN) >>Visit HPA database.

Gene(ID):

CHAT(1103)

Description:

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Sequence:

P28329 CLAT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGLRTAKKRGLGGGGKWKREEGGGTRGRREVRPACFLQSGGRGDPGDVGGPAGNPGCSPHPRAATRPPPLPAHTPAHTPEWCGAASAEAAEPRRAGPHLCIPAPGLTKTPILEKVPRKMAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHVTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

PTMs - P28329 As Substrate

P28329

Site	PTM Type	Enzyme	Source
K7	Acetylation		Uniprot
K16	Acetylation		Uniprot
K18	Acetylation		Uniprot
T25	Phosphorylation		Uniprot
T123	Phosphorylation		Uniprot
S125	Phosphorylation		Uniprot
S237	Phosphorylation		Uniprot
S244	Phosphorylation		Uniprot
T373	Phosphorylation	P17252 (PRKCA) , P05771 (PRKCB) , P05129 (PRKCG)	Uniprot
S377	Phosphorylation		Uniprot
T462	Phosphorylation		Uniprot
S464	Phosphorylation	Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)	Uniprot
S465	Phosphorylation	P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB) , Q02156 (PRKCE)	Uniprot
S473	Phosphorylation		Uniprot
S475	Phosphorylation		Uniprot
T553	Phosphorylation		Uniprot
Y554	Phosphorylation		Uniprot
S558	Phosphorylation	Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB) , Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG)	Uniprot
T574	Phosphorylation	Q9UQM7 (CAMK2A) , Q13555 (CAMK2G)	Uniprot
S594	Phosphorylation	Q02156 (PRKCE) , P05129 (PRKCG) , Q05513 (PRKCZ) , P17252 (PRKCA) , Q05655 (PRKCD) , P05771 (PRKCB)	Uniprot

Research Backgrounds

P28329_CLAT_HUMAN

Function:

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Family&Domains:

Belongs to the carnitine/choline acetyltransferase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Organismal Systems > Nervous system > Cholinergic synapse.

References

1). Neural stem cell transplantation improves learning and memory by protecting cholinergic neurons and restoring synaptic impairment in an amyloid precursor protein/presenilin 1 transgenic mouse model of Alzheimer's disease. Molecular Medicine Reports, 2020 (PubMed: 31922229) [IF=3.4]

Application: WB Species: mouse Sample: basal forebrain and hippocampus

Figure 5. |Cholinergic neurons in the basal forebrain. (A) Confocal display the ChAT‑positive neurons, (A‑a) WT group; (A‑b) Tg‑AD group; (A‑c) Tg‑NSC group. (B and C) ChAT protein expression in the basal forebrain and hippocampus.

Application: IF/ICC Species: mouse Sample: basal forebrain

2). Transplantation of NEP1-40 and NT-3 Gene-Co-Transduced Neural Stem Cells Improves Function and Neurogenesis after Spinal Cord Injury in a Rat Model. NEUROLOGY INDIA, 2022 (PubMed: 36412377) [IF=2.7]

Application: IHC Species: Rat Sample:

Figure 4: (a) Expression of ChAT at the SCI lesion in the six groups as determined by immunohistochemical staining (×200 original magnification). (b) OD intensity of ChAT expression in all groups. (c) Expression of GAD67 at the SCI lesion in the six groups according to immunohistochemical staining (×200 original magnification). (d) OD intensity of GAD67 expression in all groups. *P < 0.05 as compared with the selected group, **P < 0.01 as compared with the selected group

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Choline Acetyltransferase Antibody - #DF6964