POLR2L Antibody | Affinity Biosciences

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Western blot analysis of extracts from Raji , using POLR2L antibody.

DF6960 at 1/100 staining Mouse liver tissue by IHC-P.

Product:	POLR2L Antibody
Catalog:	DF6960
Description:	Rabbit polyclonal antibody to POLR2L
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Chicken
Mol.Wt.:	7kDa; 8kD(Calculated).
Uniprot:	P62875
RRID:	AB_2838916

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

POLR2L Antibody detects endogenous levels of total POLR2L.

RRID:

AB_2838916
Cite Format: Affinity Biosciences Cat# DF6960, RRID:AB_2838916.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

and III subunit ABC5; and III subunit RPABC5; DNA directed RNA polymerase II polypeptide L; DNA-directed RNA polymerase III subunit L; DNA-directed RNA polymerases I; hRPB7.6; hsRPB10b; II; POLR2L; RNA polymerase II 7.6 kDa subunit; RNA polymerase II polypeptide L; RNA polymerases I; RPAB5_HUMAN; RPABC5; RPB10; RPB10 homolog; RPB10beta; RPB7.6;

Immunogens

Immunogen:

Uniprot:

P62875(RPAB5_HUMAN) >>Visit HPA database.

Gene(ID):

POLR2L(5441)

Description:

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases.

Sequence:

P62875 RPAB5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNYAPLEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Zebrafish

100

Chicken

100

Horse

Sheep

Dog

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62875 As Substrate

P62875

Site	PTM Type	Source
K12	Ubiquitination	Uniprot
K17	Ubiquitination	Uniprot
K41	Ubiquitination	Uniprot
K58	Ubiquitination	Uniprot
Y62	Phosphorylation	Uniprot
K67	Ubiquitination	Uniprot

Research Backgrounds

P62875_RPAB5_HUMAN

Function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2L/RBP10 is part of the core element with the central large cleft (By similarity).

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.

Family&Domains:

Belongs to the archaeal RpoN/eukaryotic RPB10 RNA polymerase subunit family.

Research Fields

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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POLR2L Antibody - #DF6960