UHRF2 Antibody - #DF6930

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Product: UHRF2 Antibody
Catalog: DF6930
Description: Rabbit polyclonal antibody to UHRF2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 90kDa; 90kD(Calculated).
Uniprot: Q96PU4
RRID: AB_2838889

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:20-1:50
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
UHRF2 Antibody detects endogenous levels of total UHRF2.
RRID:
AB_2838889
Cite Format: Affinity Biosciences Cat# DF6930, RRID:AB_2838889.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp434B0920; DKFZp686G0837; E3 ubiquitin-protein ligase UHRF2; MGC33463; Np95 like RING finger protein; Np95-like ring finger protein; Np95/ICBP90 like RING finger protein; Np95/ICBP90-like RING finger protein; Nuclear protein 97; Nuclear zinc finger protein Np97; RING finger protein 107; RNF 107; RP11-472F14.2; Ubiquitin like containing PHD and RING finger domains protein 2; Ubiquitin-like PHD and RING finger domain-containing protein 2; Ubiquitin-like-containing PHD and RING finger domains protein 2; Uhrf2; UHRF2_HUMAN; URF 2;

Immunogens

Immunogen:
Uniprot:

Q96PU4(UHRF2_HUMAN) >>Visit HPA database.

Gene(ID):
UHRF2(115426)
Description:
This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding. [provided by RefSeq, Feb 2012]
Sequence:
MWIQVRTIDGSKTCTIEDVSRKATIEELRERVWALFDVRPECQRLFYRGKQLENGYTLFDYDVGLNDIIQLLVRPDPDHLPGTSTQIEAKPCSNSPPKVKKAPRVGPSNQPSTSARARLIDPGFGIYKVNELVDARDVGLGAWFEAHIHSVTRASDGQSRGKTPLKNGSSCKRTNGNIKHKSKENTNKLDSVPSTSNSDCVAADEDVIYHIQYDEYPESGTLEMNVKDLRPRARTILKWNELNVGDVVMVNYNVESPGQRGFWFDAEITTLKTISRTKKELRVKIFLGGSEGTLNDCKIISVDEIFKIERPGAHPLSFADGKFLRRNDPECDLCGGDPEKKCHSCSCRVCGGKHEPNMQLLCDECNVAYHIYCLNPPLDKVPEEEYWYCPSCKTDSSEVVKAGERLKMSKKKAKMPSASTESRRDWGRGMACVGRTRECTIVPSNHYGPIPGIPVGSTWRFRVQVSEAGVHRPHVGGIHGRSNDGAYSLVLAGGFADEVDRGDEFTYTGSGGKNLAGNKRIGAPSADQTLTNMNRALALNCDAPLDDKIGAESRNWRAGKPVRVIRSFKGRKISKYAPEEGNRYDGIYKVVKYWPEISSSHGFLVWRYLLRRDDVEPAPWTSEGIERSRRLCLRLQYPAGYPSDKEGKKPKGQSKKQPSGTTKRPISDDDCPSASKVYKASDSAEAIEAFQLTPQQQHLIREDCQNQKLWDEVLSHLVEGPNFLKKLEQSFMCVCCQELVYQPVTTECFHNVCKDCLQRSFKAQVFSCPACRHDLGQNYIMIPNEILQTLLDLFFPGYSKGR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Chicken
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96PU4 As Substrate

Site PTM Type Enzyme
K12 Acetylation
K12 Ubiquitination
K22 Ubiquitination
S95 Phosphorylation
S108 Phosphorylation
K128 Ubiquitination
K238 Sumoylation
S290 Phosphorylation
K307 Ubiquitination
K322 Ubiquitination
K393 Ubiquitination
K401 Sumoylation
K401 Ubiquitination
R428 Methylation
K519 Ubiquitination
K548 Ubiquitination
K575 Ubiquitination
Y584 Phosphorylation
Y588 Phosphorylation
K589 Ubiquitination
Y641 Phosphorylation
S643 Phosphorylation
S654 Phosphorylation
S659 Phosphorylation
T661 Phosphorylation
T662 Phosphorylation
K663 Ubiquitination
S667 Phosphorylation
S673 Phosphorylation
K676 Ubiquitination
K679 Ubiquitination
S683 Phosphorylation
K708 Ubiquitination

Research Backgrounds

Function:

E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. E3 SUMO-, but not ubiquitin-, protein ligase for ZNF131.

PTMs:

May be autoubiquitinated; which may lead to proteasomal degradation.

Phosphorylated. Phosphorylation may be mediated by CDK2.

Autosumoylated.

Subcellular Location:

Nucleus.
Note: Enriched at pericentric heterochromatin (PH). This localization is dependent on the interaction with H3K9me3 (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; disulfide-linked. Binds methylated CpG containing oligonucleotides. Interacts with H3: the interaction has a preference for the 'Lys-9' trimethylated form of H3 (H3K9me3) (By similarity). Interacts with PCNP, HDAC1 and CDK2 (inactive form). Component of a complex at least composed of UHRF2, CDK2 and CCNE1. Interacts directly with CCNE1; the interaction ubiquitinates CCNE1 and appears independent of CCNE1 phosphorylation. Interacts with CCND1; the interaction ubiquitinates CCND1 and appears independent of CCND1 phosphorylation. Interacts with p53/TP53 and RB1. Interacts with UBE2I.

References

1). The prognostic value of ubiquitin/ubiquitin-like-related genes along with immune cell infiltration and clinicopathological features in osteosarcoma. Journal of orthopaedic surgery and research, 2024 (PubMed: 38879525) [IF=2.8]

Application: WB    Species: Human    Sample:

Fig. 8 The mRNA and protein expression of TRIM8 and UHRF2 in cell lines. The mRNA expression of TRIM8 (A) and UHRF2 (B) relative to that of β-actin in osteosarcoma and osteoblast cells by qRT-PCR (2−ΔΔCT method, mean ± standard error of the mean). C Western blotting analysis of TRIM8 and UHRF2 expression. The protein expression of TRIM8 (D) and UHRF2 (E) relative to that of β-actin in osteosarcoma and osteoblast cells was determined by Western blotting analysis (mean ± standard error of the mean)

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