NOX4 Antibody - #DF6924

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Product: NOX4 Antibody
Catalog: DF6924
Description: Rabbit polyclonal antibody to NOX4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 67kDa; 67kD(Calculated).
Uniprot: Q9NPH5
RRID: AB_2838883

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
NOX4 Antibody detects endogenous levels of total NOX4.
RRID:
AB_2838883
Cite Format: Affinity Biosciences Cat# DF6924, RRID:AB_2838883.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Kidney oxidase 1; Kidney oxidase-1; Kidney superoxide producing NADPH oxidase; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; KOX1; NADPH; NADPH oxidase 4; Nox4; NOX4_HUMAN; Renal NAD(P)H oxidase; Renal NAD(P)H-oxidase; RENOX;

Immunogens

Immunogen:
Uniprot:

Q9NPH5(NOX4_HUMAN) >>Visit HPA database.

Gene(ID):
NOX4(50507)
Expression:
Q9NPH5 NOX4_HUMAN:

Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

Description:
NOX4 (NADPH oxidase 4 ) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex.NOX4 has 7 isforms and the molecular weight are 7KDa,26-32KDa,58-67KDa,75-80KDa(PMID:11728818).This antibody is specific to NOX4.
Sequence:
MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRASASVLNLNCSLILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSRTFHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFVELNAARYRDEDPRKLLFTTVPGLTGVCMVVVLFLMITASTYAIRVSNYDIFWYTHNLFFVFYMLLTLHVSGGLLKYQTNLDTHPPGCISLNRTSSQNISLPEYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANFPQTWLWISGPLCLYCAERLYRYIRSNKPVTIISVMSHPSDVMEIRMVKENFKARPGQYITLHCPSVSALENHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
91
Chicken
82
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPH5 As Substrate

Site PTM Type Enzyme
Y491 Phosphorylation
Y566 Phosphorylation

Research Backgrounds

Function:

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.

Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.

PTMs:

Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cell junction>Focal adhesion.
Note: May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.

Nucleus. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

Subunit Structure:

Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4.

References

1). Human umbilical cord mesenchymal stem cells alleviate chemotherapy-induced premature ovarian insufficiency mouse model by suppressing ferritinophagy-mediated ferroptosis in granulosa cells. Free radical biology & medicine, 2024 (PubMed: 38677487) [IF=7.1]
2). Chronic infusion of ELABELA alleviates vascular remodeling in spontaneously hypertensive rats via anti-inflammatory, anti-oxidative and anti-proliferative effects. ACTA PHARMACOLOGICA SINICA, 2022 (PubMed: 35260820) [IF=6.9]
3). Protective Role of Dioscin against Doxorubicin-Induced Chronic Cardiotoxicity: Insights from Nrf2-GPX4 Axis-Mediated Cardiac Ferroptosis. Biomolecules, 2024 (PubMed: 38672439) [IF=5.5]

Application: WB    Species: Rat    Sample:

Figure 8 Dioscin suppresses cardiac ferroptosis by regulating the Nrf2/GPX4 signaling pathway in DOX-induced rats. (A) Representative Western blotting images of Nrf2, HO-1, and TfR1 in DOX-induced rat treated with or without dioscin. (B–D) Bar charts indicate the protein expression levels of Nrf2, HO-1, and TfR1 in cardiac tissues. Data are mean ± SD (n = 3). (E–K) Bar charts indicate the mRNA expression levels of Nrf2, HO-1, TfR1, DMT1, FTL, FTH1, and FPN in cardiac tissues. Data are mean ± SD (n = 6). (L) Representative Western blotting images of NOX4, ABCB8, and FXN in DOX-induced rat treated with or without dioscin. (M–O) Bar charts indicate the protein expression levels of NOX4, ABCB8, and FXN in cardiac tissues. Data are mean ± SD (n = 3). (P–R) Bar charts indicate the mRNA expression levels of NOX4, ABCB8, and FXN in cardiac tissues. Data are mean ± SD (n = 6). (S) Representative images of immunofluorescence analysis of Nrf2 and NOX4 expression were captured by fluorescence microscopy. (T,U) The bar chart indicates the Nrf2 and NOX4 intensity in cardiac tissue. Data are mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. DOX treatment group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. control group. (A,L) Original western blotting figures can be found in Figures S5 and S6.
4). Caviunin glycoside (CAFG) from Dalbergia sissoo attenuates osteoarthritis by modulating chondrogenic and matrix regulating proteins. Journal of Ethnopharmacology, 2022 (PubMed: 34116187) [IF=4.8]
5). Geranylgeranylacetone, an inducer of heat shock protein 70, attenuates pulmonary fibrosis via inhibiting NF-κB/NOX4/ROS signalling pathway in vitro and in vivo. Chemico-Biological Interactions, 2023 (PubMed: 37307957) [IF=4.7]
6). Enhancer of zeste homolog 2 modulates oxidative stress-mediated pyroptosis in vitro and in a mouse kidney ischemia-reperfusion injury model. FASEB JOURNAL, 2020 (PubMed: 31914694) [IF=4.4]

Application: WB    Species: mouse    Sample: kidneys

FIGURE 6|EZH2 inhibition alleviated pyroptosis through Nox4. A, Representative bands of western blot analysis for the expression of Nox4 and bar graphs showed the fold changes, n = 3.
7). Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration. Disease Models & Mechanisms, 2021 (PubMed: 34821368) [IF=4.0]

Application: WB    Species: Mouse    Sample:

Fig. 4. Linear and bell-shaped dose response of reactive oxygen species (ROS) and the antioxidant system to gelatin. (A,B) Flow cytometry analysis (A) and quantification (B) of cells stained with 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA; DCF) (ROS indicator). Count (y-axis in A) indicates cell counts (n=3). (C) Production of specific ROS: O2−, ·OH and H2O2 (n=3). (D) Activities of antioxidases: superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) (n=3). (E,F) Western blots (E) and quantification (F) of NOX2 and NOX4 in the cells cultured on gelatin-coated dishes at 90% confluence (n=3). β-Actin is used as a loading control. (G) Quantification of MitoSOX+ cells obtained by flow cytometry (n=3). (H) Flow cytometry analysis of the cells treated with diphenyliodonium (DPI; 0.5 μM, NOX2 inhibitor) and rotenone (ROT; 10 μM, an inhibitor of mitochondrial respiratory complex I) (n=3). Significance was determined by unpaired two-tailed Student's t-test with Welch's correction (B-D,F-G) and one-way ANOVA with Tukey's post-hoc test (H).
8). Dapagliflozin inhibits ferroptosis and ameliorates renal fibrosis in diabetic C57BL/6J mice. Scientific reports, 2025 (PubMed: 40016517) [IF=3.8]
9). Inhibition of (Pro)renin Receptor-Mediated Oxidative Stress Alleviates Doxorubicin-Induced Heart Failure. Frontiers in Oncology, 2022 (PubMed: 35574363) [IF=3.5]

Application: WB    Species: Rat    Sample: H9C2 cells

Figure 3 PRR inhibition downregulated the overexpression of oxidant species and apoptosis in H9C2 cells treated with doxorubicin. (A) Cell viability of H9C2 cells treated with Doxorubicin (DOX) treated at different concentrations (0–10 μM). (B) The protein expression of PRR downregulated by PRR-siRNA in H9C2 cells. (B1) Quantification of PRR/GAPDH protein expression levels relative to changing PRR expression. (C) Representative oxidative stress based on DHE relative fluorescence intensity of H9C2 cells. (D) Flow cytometry shows that PRR expression is directly related to cell apoptosis in doxorubicin (DOX)-treated H9C2 cells. (E) The protein expression of RAC1, NOX2, NOX4, and cleaved caspase3 was regulated by PRR inhibition in H9C2 cells. (E1)-(E4) Quantification of RAC1/GAPDH, NOX2/GAPDH, NOX4/GAPDH, and cleaved caspase3/GAPDH protein expression levels relative to changing PRR expression. (*p < 0.05, **p < 0.01 vs the control group. # p < 0.05, ## p < 0.01vs DOX group).
10). Ebselen ameliorates renal ischemia–reperfusion injury via enhancing autophagy in rats. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2022 (PubMed: 35338455) [IF=3.5]
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