DLGAP5 Antibody - #DF6920

(1)  
Product: DLGAP5 Antibody
Catalog: DF6920
Description: Rabbit polyclonal antibody to DLGAP5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 95kDa; 95kD(Calculated).
Uniprot: Q15398
RRID: AB_2838879

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
DLGAP5 Antibody detects endogenous levels of total DLGAP5.
RRID:
AB_2838879
Cite Format: Affinity Biosciences Cat# DF6920, RRID:AB_2838879.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DAP-5; DAP5; Discs large homolog 7; Disks large associated protein 5; Disks large associated protein DLG7; Disks large-associated protein 5; Disks large-associated protein DLG7; DLG 1; DLG 7; DLG1; DLG7; DLGAP5; DLGP5_HUMAN; Drosophila discs large 1 tumor suppressor like; Hepatoma up regulated protein; Hepatoma up-regulated protein; Hepatoma upregulated protein; HURP; KIAA0008;

Immunogens

Immunogen:
Uniprot:

Q15398(DLGP5_HUMAN) >>Visit HPA database.

Gene(ID):
DLGAP5(9787)
Expression:
Q15398 DLGP5_HUMAN:

Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.

Description:
DLG7 is a potential cell cycle regulator that may play a role in carcinogenesis of cancer cells and mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. The protein is also a key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Sequence:
MSSSHFASRHRKDISTEMIRTKIAHRKSLSQKENRHKEYERNRHFGLKDVNIPTLEGRILVELDETSQGLVPEKTNVKPRAMKTILGDQRKQMLQKYKEEKQLQKLKEQREKAKRGIFKVGRYRPDMPCFLLSNQNAVKAEPKKAIPSSVRITRSKAKDQMEQTKIDNESDVRAIRPGPRQTSEKKVSDKEKKVVQPVMPTSLRMTRSATQAAKQVPRTVSSTTARKPVTRAANENEPEGKVPSKGRPAKNVETKPDKGISCKVDSEENTLNSQTNATSGMNPDGVLSKMENLPEINTAKIKGKNSFAPKDFMFQPLDGLKTYQVTPMTPRSANAFLTPSYTWTPLKTEVDESQATKEILAQKCKTYSTKTIQQDSNKLPCPLGPLTVWHEEHVLNKNEATTKNLNGLPIKEVPSLERNEGRIAQPHHGVPYFRNILQSETEKLTSHCFEWDRKLELDIPDDAKDLIRTAVGQTRLLMKERFKQFEGLVDDCEYKRGIKETTCTDLDGFWDMVSFQIEDVIHKFNNLIKLEESGWQVNNNMNHNMNKNVFRKKVVSGIASKPKQDDAGRIAARNRLAAIKNAMRERIRQEECAETAVSVIPKEVDKIVFDAGFFRVESPVKLFSGLSVSSEGPSQRLGTPKSVNKAVSQSRNEMGIPQQTTSPENAGPQNTKSEHVKKTLFLSIPESRSSIEDAQCPGLPDLIEENHVVNKTDLKVDCLSSERMSLPLLAGGVADDINTNKKEGISDVVEGMELNSSITSQDVLMSSPEKNTASQNSILEEGETKISQSELFDNKSLTTECHLLDSPGLNCSNPFTQLERRHQEHARHISFGGNLITFSPLQPGEF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Chicken
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15398 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
S15 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
S67 Phosphorylation
T84 Phosphorylation
K158 Methylation
T182 Phosphorylation
S188 Phosphorylation
K192 Acetylation
K193 Sumoylation
T201 Phosphorylation
S202 Phosphorylation
R204 Methylation
T219 Phosphorylation
S221 Phosphorylation
T224 Phosphorylation
S306 Phosphorylation
K310 Ubiquitination
T326 Phosphorylation
T329 Phosphorylation P28482 (MAPK1) , P06493 (CDK1)
S332 Phosphorylation
T338 Phosphorylation
S340 Phosphorylation
T342 Phosphorylation P24941 (CDK2)
T344 Phosphorylation P24941 (CDK2)
K347 Sumoylation
K347 Ubiquitination
T348 Phosphorylation
S353 Phosphorylation
T356 Phosphorylation
K357 Ubiquitination
K363 Ubiquitination
S368 Phosphorylation
K370 Ubiquitination
S376 Phosphorylation
K397 Ubiquitination
T401 Phosphorylation
T402 Phosphorylation
K403 Ubiquitination
K411 Ubiquitination
S415 Phosphorylation
T441 Phosphorylation
K443 Ubiquitination
K454 Ubiquitination
K464 Ubiquitination
T469 Phosphorylation
K483 Ubiquitination
K495 Ubiquitination
K529 Ubiquitination
K547 Ubiquitination
K553 Ubiquitination
K561 Ubiquitination
K580 Ubiquitination
S618 Phosphorylation P06493 (CDK1)
S624 Phosphorylation
S627 Phosphorylation O14965 (AURKA)
S629 Phosphorylation
S630 Phosphorylation
S634 Phosphorylation
T639 Phosphorylation P06493 (CDK1)
S642 Phosphorylation
K645 Ubiquitination
T661 Phosphorylation
S662 Phosphorylation
T671 Phosphorylation
S687 Phosphorylation
S689 Phosphorylation
S690 Phosphorylation
K711 Ubiquitination
K715 Ubiquitination
S720 Phosphorylation
S721 Phosphorylation
S725 Phosphorylation O14965 (AURKA)
S757 Phosphorylation O14965 (AURKA)
T759 Phosphorylation
S767 Phosphorylation
S774 Phosphorylation
S777 Phosphorylation
T784 Phosphorylation
K785 Ubiquitination
S787 Phosphorylation
S789 Phosphorylation
S796 Phosphorylation
T798 Phosphorylation
T799 Phosphorylation
S806 Phosphorylation
S812 Phosphorylation
T816 Phosphorylation
S830 Phosphorylation O14965 (AURKA)
T837 Phosphorylation
S839 Phosphorylation P06493 (CDK1)

Research Backgrounds

Function:

Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.

PTMs:

Ubiquitinated, leading to its degradation.

Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle.
Note: Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.

Subunit Structure:

Interacts with CDK1. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDK1/Cyclin B-phosphorylation dependent manner. Interacts with CDH1.

Family&Domains:

Belongs to the SAPAP family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.