PSMA6 Antibody - #DF6911

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Product: PSMA6 Antibody
Catalog: DF6911
Description: Rabbit polyclonal antibody to PSMA6
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 27kDa; 27kD(Calculated).
Uniprot: P60900
RRID: AB_2838870

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PSMA6 Antibody detects endogenous levels of total PSMA6.
RRID:
AB_2838870
Cite Format: Affinity Biosciences Cat# DF6911, RRID:AB_2838870.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

27 kDa prosomal protein; IOTA; Macropain iota chain; Macropain subunit iota; MGC22756; MGC2333; MGC23846; Multicatalytic endopeptidase complex iota chain; p27K; PROS 27; PROS-27; PROS27; Prosomal P27K protein; Proteasome (prosome macropain) subunit alpha type 6; Proteasome iota chain; Proteasome subunit alpha type 6; Proteasome subunit alpha type-6; Proteasome subunit iota; PSA6_HUMAN; PSMA 6; PSMA6;

Immunogens

Immunogen:
Uniprot:

P60900(PSA6_HUMAN) >>Visit HPA database.

Gene(ID):
PSMA6(5687)
Description:
The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of α subunits (called PSMAs), and the two inner layers are identical rings composed of β subunits. While the catalytic sites are located on the β rings (1-3), the α subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different α and ten different β proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9).
Sequence:
MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60900 As Substrate

Site PTM Type Enzyme
R3 Methylation
S5 Phosphorylation
S6 Phosphorylation
R11 Methylation
T14 Phosphorylation
S17 Phosphorylation
R21 Methylation
Y23 Phosphorylation
Y27 Phosphorylation
K30 Methylation
K30 Ubiquitination
K45 Acetylation
K45 Ubiquitination
K54 Ubiquitination
K55 Acetylation
K55 Sumoylation
K55 Ubiquitination
K59 Acetylation
K59 Ubiquitination
S63 Phosphorylation
S64 Phosphorylation
T65 Phosphorylation
K71 Ubiquitination
T73 Phosphorylation
Y96 Phosphorylation
K102 Acetylation
K102 Methylation
K102 Sumoylation
K102 Ubiquitination
Y103 Phosphorylation
K104 Acetylation
K104 Ubiquitination
Y105 Phosphorylation
Y107 Phosphorylation
K116 Acetylation
K116 Ubiquitination
K153 Ubiquitination
C154 S-Nitrosylation
Y159 Phosphorylation
Y160 Phosphorylation
C161 S-Nitrosylation
K164 Ubiquitination
K171 Ubiquitination
K181 Acetylation
K181 Ubiquitination
K182 Ubiquitination
K211 Ubiquitination
S213 Phosphorylation
T231 Phosphorylation
R245 Methylation

Research Backgrounds

Function:

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Colocalizes with TRIM5 in cytoplasmic bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Interacts with ALKBH4.

Family&Domains:

Belongs to the peptidase T1A family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

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