PLAU Antibody | Affinity Biosciences

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Cites

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Western blot analysis of extracts from COS-7, using PLAU Antibody.

d Cell lysates were electrophoresed and MMP- 2/9 and uPA proteins were detected by their respective specific antibodies in indicated concentrations.

Figure 4 LrB suppressed the expression of PAI-1 and the activation of TGF-β1/Smad signaling pathway in MI/R mice.

Product:	PLAU Antibody
Catalog:	DF6904
Description:	Rabbit polyclonal antibody to PLAU
Application:	WB IHC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Horse, Rabbit
Mol.Wt.:	49kDa, 57 kDa; 49kD(Calculated).
Uniprot:	P00749
RRID:	AB_2838863

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Horse(82%), Rabbit(82%)

Clonality:

Polyclonal

Specificity:

PLAU Antibody detects endogenous levels of total PLAU.

RRID:

AB_2838863
Cite Format: Affinity Biosciences Cat# DF6904, RRID:AB_2838863.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATF; ATF uPA; BDPLT5; Plasminogen activator; Plasminogen activator urinary; Plasminogen activator urokinase; PLAU; QPD; u PA; U plasminogen activator; u-PA; U-plasminogen activator; uPA; URK; UROK_HUMAN; Urokinase plasminogen activator; Urokinase type plasminogen activator; Urokinase type plasminogen activator precursor; Urokinase-type plasminogen activator chain B;

Immunogens

Immunogen:

Uniprot:

P00749(UROK_HUMAN) >>Visit HPA database.

Gene(ID):

PLAU(5328)

Expression:

P00749 UROK_HUMAN:

Expressed in the prostate gland and prostate cancers.

Description:

The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65 kDa, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35 kDa) (1-3). It is a central player in the plasminogen activation pathway. uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4). Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5). Therefore, uPAR plays a key role in regulating extracellular proteolysis. In addition, uPAR plays an important role in regulating cell proliferation, adhesion, and mobility (6,7). Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9).

Sequence:

P00749 UROK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRALLARLLLCVLVVSDSKGSNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKPLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Horse

Bovine

Sheep

Pig

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00749 As Substrate

P00749

Site	PTM Type	Enzyme	Source
T38	O-Glycosylation		Uniprot
T38	Phosphorylation		Uniprot
S41	Phosphorylation		Uniprot
S158	O-Glycosylation		Uniprot
S158	Phosphorylation	P17252 (PRKCA)	Uniprot
S323	Phosphorylation	P17252 (PRKCA)	Uniprot
K424	Ubiquitination		Uniprot

Research Backgrounds

P00749_UROK_HUMAN

Function:

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

PTMs:

Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed in the prostate gland and prostate cancers.

Subunit Structure:

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR. In complex with SERPINE1, interacts with PLAUR/uPAR. Interacts with SORL1 and LRP1, either alone or in complex with SERPINE1; these interactions are abolished in the presence of LRPAP1/RAP. The ternary complex composed of PLAUR-PLAU-PAI1 also interacts with SORLA.

Family&Domains:

Belongs to the peptidase S1 family.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). Fucoxanthin Activates Apoptosis via Inhibition of PI3K/Akt/mTOR Pathway and Suppresses Invasion and Migration by Restriction of p38-MMP-2/9 Pathway in Human Glioblastoma Cells. NEUROCHEMICAL RESEARCH, 2016 (PubMed: 27394418) [IF=4.4]

Application: WB Species: human Sample:

d Cell lysates were electrophoresed and MMP- 2/9 and uPA proteins were detected by their respective specific antibodies in indicated concentrations

2). Loureirin B Alleviates Myocardial Ischemia/Reperfusion Injury via Inhibiting PAI-1/TGF-β1/Smad Signaling Pathway. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35535157)

Application: WB Species: Mice Sample:

Figure 4 LrB suppressed the expression of PAI-1 and the activation of TGF-β1/Smad signaling pathway in MI/R mice. (a) Representative images showing the distribution of PAI-1 in cardiac tissues after immunofluorescence staining. Scar bar = 50 μm. (b, c) Relative mRNA and protein expression of urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in the cardiac tissues. (d) Western blot analysis for TGF-β1R, TGF-β1, p-Smad2/3, and Smad2/3. Relative protein expression of (e) PAI-1, (f) TGF-β1R, (g) TGF-β1, (h) p-Smad2/3, and (i) Smad2/3 in the cardiac tissues. Values are expressed as mean ± SD (n = 6). +++p < 0.001vs. sham group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001vs. MI/R group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PLAU Antibody - #DF6904