MSN Antibody - #DF6901

(2)  
Product: MSN Antibody
Catalog: DF6901
Description: Rabbit polyclonal antibody to MSN
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 68kDa; 68kD(Calculated).
Uniprot: P26038
RRID: AB_2838860

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MSN Antibody detects endogenous levels of total MSN.
RRID:
AB_2838860
Cite Format: Affinity Biosciences Cat# DF6901, RRID:AB_2838860.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Epididymis luminal protein 70; HEL70; Membrane organizing extension spike protein; Membrane-organizing extension spike protein; MOES_HUMAN; Moesin; Moesin/anaplastic lymphoma kinase fusion protein; Msn; MSN/ALK fusion;

Immunogens

Immunogen:
Uniprot:

P26038(MOES_HUMAN) >>Visit HPA database.

Gene(ID):
MSN(4478)
Expression:
P26038 MOES_HUMAN:

In all tissues and cultured cells studied.

Description:
The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).
Sequence:
MPKTISVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWFFGLQYQDTKGFSTWLKLNKKVTAQDVRKESPLLFKFRAKFYPEDVSEELIQDITQRLFFLQVKEGILNDDIYCPPETAVLLASYAVQSKYGDFNKEVHKSGYLAGDKLLPQRVLEQHKLNKDQWEERIQVWHEEHRGMLREDAVLEYLKIAQDLEMYGVNYFSIKNKKGSELWLGVDALGLNIYEQNDRLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQMERAMLENEKKKREMAEKEKEKIEREKEELMERLKQIEEQTKKAQQELEEQTRRALELEQERKRAQSEAEKLAKERQEAEEAKEALLQASRDQKKTQEQLALEMAELTARISQLEMARQKKESEAVEWQQKAQMVQEDLEKTRAELKTAMSTPHVAEPAENEQDEQDENGAEASADLRADAMAKDRSEEERTTEAEKNERVQKHLKALTSELANARDESKKTANDMIHAENMRLGRDKYKTLRQIRQGNTKQRIDEFESM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26038 As Substrate

Site PTM Type Enzyme
K3 Ubiquitination
T4 Phosphorylation
K35 Acetylation
K35 Ubiquitination
S56 Phosphorylation
T57 Phosphorylation
K60 Acetylation
K64 Acetylation
K64 Ubiquitination
T66 Phosphorylation P34947 (GRK5)
R71 Methylation
K72 Ubiquitination
S74 Phosphorylation
K79 Acetylation
K79 Ubiquitination
K83 Acetylation
K83 Ubiquitination
T98 Phosphorylation
K107 Ubiquitination
Y116 Phosphorylation
S127 Phosphorylation
S132 Phosphorylation
K133 Acetylation
K139 Acetylation
K139 Ubiquitination
K143 Ubiquitination
S144 Phosphorylation
Y146 Phosphorylation
K151 Acetylation
K151 Ubiquitination
K162 Acetylation
K162 Ubiquitination
K165 Acetylation
K165 Ubiquitination
Y191 Phosphorylation
Y205 Phosphorylation
S207 Phosphorylation
K209 Ubiquitination
K211 Ubiquitination
K212 Ubiquitination
Y228 Phosphorylation
K237 Ubiquitination
S243 Phosphorylation
S249 Phosphorylation
K253 Acetylation
K253 Ubiquitination
K254 Ubiquitination
K258 Acetylation
K258 Ubiquitination
K262 Acetylation
K262 Sumoylation
K262 Ubiquitination
K263 Acetylation
K263 Ubiquitination
Y270 Phosphorylation
R273 Methylation
Y291 Phosphorylation
K296 Ubiquitination
T299 Phosphorylation
K306 Methylation
K306 Ubiquitination
K327 Acetylation
K344 Acetylation
K344 Ubiquitination
K352 Ubiquitination
K360 Ubiquitination
K380 Ubiquitination
S384 Phosphorylation
K388 Acetylation
K388 Ubiquitination
K391 Ubiquitination
K400 Acetylation
K400 Ubiquitination
S407 Phosphorylation
K412 Ubiquitination
T413 Phosphorylation
T425 Phosphorylation
S429 Phosphorylation
S440 Phosphorylation
K448 Ubiquitination
K458 Ubiquitination
K464 Ubiquitination
T465 Phosphorylation
S468 Phosphorylation
T469 Phosphorylation
S491 Phosphorylation
S504 Phosphorylation
K520 Ubiquitination
K523 Ubiquitination
T526 Phosphorylation
S527 Phosphorylation
S536 Phosphorylation
K537 Ubiquitination
K538 Ubiquitination
Y556 Phosphorylation
T558 Phosphorylation O95819 (MAP4K4) , Q5VT25 (CDC42BPA) , P41279 (MAP3K8) , Q04759 (PRKCQ) , O75116 (ROCK2) , Q5S007 (LRRK2) , P25098 (GRK2) , P17612 (PRKACA) , Q13464 (ROCK1) , Q9Y5S2 (CDC42BPB) , O94804 (STK10)
T567 Phosphorylation
S576 Phosphorylation

Research Backgrounds

Function:

Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton. Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement. These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction. The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs. Modulates phagolysosomal biogenesis in macrophages (By similarity). Participates also in immunologic synapse formation.

PTMs:

Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization.

S-nitrosylation of Cys-117 is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) implicating the iNOS-S100A8/9 transnitrosylase complex.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton. Apical cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Microvillus membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Microvillus.
Note: Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By similarity). Localizes at the uropods of T lymphoblasts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In all tissues and cultured cells studied.

Subunit Structure:

In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Interacts with SLC9A3R1. Interacts with PPP1R16B. Interacts with PDZD8. Interacts with SELPLG and SYK; these interaction mediate the activation of SYK by SELPLG. Interacts with PDPN (via cytoplasmic domain); this interaction activates RHOA and promotes epithelial-mesenchymal transition. Interacts with SPN/CD43. Interacts with CD44. Interacts with ICAM2 (By similarity). Interacts with ICAM3 (via C-terminus). Interacts with PDZD8. Interacts with F-actin. Interacts with CD46. Interacts with PTPN6 (By similarity).

(Microbial infection) Interacts with HIV-1 envelope protein gp120.

Family&Domains:

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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