IDH1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using IDH1 Antibody.

DF6892 at 1/100 staining Mouse brain tissue by IHC-P.

DF6892 at 1/100 staining Mouse testis tissue by IHC-P.

DF6892 at 1/100 staining Rat brain tissue by IHC-P.

DF6892 at 1/100 staining Rat testis tissue by IHC-P.

Product:	IDH1 Antibody
Catalog:	DF6892
Description:	Rabbit polyclonal antibody to IDH1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	47kDa; 47kD(Calculated).
Uniprot:	O75874
RRID:	AB_2838851

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

IDH1 Antibody detects endogenous levels of total IDH1.

RRID:

AB_2838851
Cite Format: Affinity Biosciences Cat# DF6892, RRID:AB_2838851.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cytosolic NADP isocitrate dehydrogenase; Cytosolic NADP-isocitrate dehydrogenase; Epididymis luminal protein 216; Epididymis secretory protein Li 26; HEL-216; HEL-S-26; ICDH; IDCD; IDH; IDH1; IDHC_HUMAN; IDP; IDPC; Isocitrate dehydrogenase [NADP] cytoplasmic; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP dependent isocitrate dehydrogenase cytosolic; NADP dependent isocitrate dehydrogenase peroxisomal; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; PICD;

Immunogens

Immunogen:

Uniprot:

O75874(IDHC_HUMAN) >>Visit HPA database.

Gene(ID):

IDH1(3417)

Description:

IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1). IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3). IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma (4). IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (5).

Sequence:

O75874 IDHC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Rabbit

100

Sheep

Chicken

Xenopus

Zebrafish

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75874 As Substrate

O75874

Site	PTM Type	Source
S2	Acetylation	Uniprot
T19	Phosphorylation	Uniprot
Y34	Phosphorylation	Uniprot
S41	Phosphorylation	Uniprot
Y42	Phosphorylation	Uniprot
K58	Acetylation	Uniprot
K58	Ubiquitination	Uniprot
K65	Ubiquitination	Uniprot
K72	Ubiquitination	Uniprot
C73	S-Nitrosylation	Uniprot
T75	Phosphorylation	Uniprot
T77	Phosphorylation	Uniprot
K81	Acetylation	Uniprot
K81	Ubiquitination	Uniprot
K87	Acetylation	Uniprot
K87	Ubiquitination	Uniprot
K93	Acetylation	Uniprot
S94	Phosphorylation	Uniprot
T106	Phosphorylation	Uniprot
C114	S-Nitrosylation	Uniprot
K126	Acetylation	Uniprot
K126	Ubiquitination	Uniprot
Y135	Phosphorylation	Uniprot
Y139	Phosphorylation	Uniprot
K151	Acetylation	Uniprot
K151	Ubiquitination	Uniprot
Y156	Phosphorylation	Uniprot
S159	Phosphorylation	Uniprot
T162	Phosphorylation	Uniprot
S188	Phosphorylation	Uniprot
Y208	Phosphorylation	Uniprot
K212	Ubiquitination	Uniprot
T214	Phosphorylation	Uniprot
Y219	Phosphorylation	Uniprot
K224	Acetylation	Uniprot
K224	Ubiquitination	Uniprot
Y231	Phosphorylation	Uniprot
K233	Acetylation	Uniprot
K233	Ubiquitination	Uniprot
K236	Ubiquitination	Uniprot
S237	Phosphorylation	Uniprot
K243	Acetylation	Uniprot
K243	Ubiquitination	Uniprot
S261	Phosphorylation	Uniprot
Y319	Phosphorylation	Uniprot
K321	Acetylation	Uniprot
K321	Ubiquitination	Uniprot
T325	Phosphorylation	Uniprot
K350	Ubiquitination	Uniprot
C379	S-Nitrosylation	Uniprot
K381	Ubiquitination	Uniprot
S389	Phosphorylation	Uniprot
Y391	Phosphorylation	Uniprot
K400	Ubiquitination	Uniprot
K406	Ubiquitination	Uniprot
K408	Ubiquitination	Uniprot

Research Backgrounds

O75874_IDHC_HUMAN

PTMs:

Acetylation at Lys-374 dramatically reduces catalytic activity.

Subcellular Location:

Cytoplasm>Cytosol. Peroxisome.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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IDH1 Antibody - #DF6892