Cytochrome P450 19A1 Antibody | Affinity Biosciences

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Cytochrome P450 19A1 Antibody - Figure 1 Estrogen levels in the circulation and brain of mice.

Cytochrome P450 19A1 Antibody - FIGURE 5 EPC regulated the lipid metabolism-related genes and proteins inMAFLD rats.

Product:	Cytochrome P450 19A1 Antibody
Catalog:	DF6884
Description:	Rabbit polyclonal antibody to Cytochrome P450 19A1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	58kDa; 58kD(Calculated).
Uniprot:	P11511
RRID:	AB_2838843

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(88%), Sheep(88%), Rabbit(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

Cytochrome P450 19A1 Antibody detects endogenous levels of total Cytochrome P450 19A1.

RRID:

AB_2838843
Cite Format: Affinity Biosciences Cat# DF6884, RRID:AB_2838843.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARO; ARO1; Aromatase; CP19A_HUMAN; CPV1; CYAR; CYP19; Cyp19a1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Cytochrome P450, family 19, subfamily A, polypeptide 1; Cytochrome P450, subfamily XIX (aromatization of androgens); Estrogen synthase; Estrogen synthetase; Flavoprotein linked monooxygenase; MGC104309; Microsomal monooxygenase; OTTHUMP00000162543; OTTHUMP00000198350; P 450AROM;

Immunogens

Immunogen:

Uniprot:

P11511(CP19A_HUMAN) >>Visit HPA database.

Gene(ID):

CYP19A1(1588)

Expression:

P11511 CP19A_HUMAN:

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

Description:

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510). This antibody is specific to CYP19A1.

Sequence:

P11511 CP19A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGIGSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPELWKTTRPFFMKALSGPGLVRMVTVCAESLKTHLDRLEEVTNESGYVDVLTLLRRVMLDTSNTLFLRIPLDESAIVVKIQGYFDAWQALLIKPDIFFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFATELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDIQKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAKNVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEMIFTPRNSDRCLEH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Bovine

Sheep

Dog

Pig

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11511 As Substrate

P11511

Site	PTM Type	Enzyme	Source
S118	Phosphorylation		Uniprot
T162	Phosphorylation		Uniprot
S167	Phosphorylation		Uniprot
T201	Phosphorylation		Uniprot
Y220	Phosphorylation		Uniprot
Y241	Phosphorylation		Uniprot
Y361	Phosphorylation	P12931 (SRC)	Uniprot
T392	Phosphorylation		Uniprot
Y441	Phosphorylation		Uniprot
T484	Phosphorylation		Uniprot
S497	Phosphorylation		Uniprot

Research Backgrounds

P11511_CP19A_HUMAN

Function:

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

PTMs:

Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Microsome membrane>Multi-pass membrane protein.

Tissue Specificity:

Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

References

1). Effects of Poria cocos extract on metabolic dysfunction-associated fatty liver disease via the FXR/PPARα-SREBPs pathway. Frontiers in Pharmacology, 2022 (PubMed: 36278226) [IF=5.6]

Application: WB Species: Rat Sample:

FIGURE 5 EPC regulated the lipid metabolism-related genes and proteins inMAFLD rats. (A) mRNA abundances of FASN. (B) mRNA abundances of PPARα (PPARΑ). (C) mRNA abundances of PPARγ (PPARG). (D) mRNA abundances of RXRα. (E) mRNA abundances of CYP19A1. (F) mRNA abundances of NR3C1. (G) mRNA abundances of SREBP-1c. (H) mRNA abundances of HMGCR. (I) mRNA abundances of SCD. n = 6; (J) Relative expression of protein SCD. (K) Relative expression of protein PPARα. (L) Relative expression of protein FASN. (M) Relative expression of protein p-JNK. (N) Relative expression of protein p-NF-κB. (O) Relative expression of protein CYP19A1. (P) Relative expression of protein NR3C1. (Q-R) Representative immunoblotting images of β-actin, SCD, PPARα, FASN,CYP19A1, NR3C1, JNK, p-JNK, NF-κB, pNF-κB; n = 4, data are presented as mean ± SEM. One-way analysis of variance (ANOVA) was conducted for the group comparison. *p < 0.05, **p < 0.01, ***p < 0.001 vs MOD group. FASN, fatty acid synthase; PPARα/γ, peroxisome proliferator-activated receptor alpha/gamma; RXRa, retinoic acid receptor alpha; SREBP-1c, sterol regulatory element binding protein 1c; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; SCD, Stearoyl-CoA desaturase; p-JNK, phosphorylation (p) -stress-activated protein kinase JNK; NF-κB, nuclear factor kappa B.

2). Acute heat stress regulates estradiol synthesis in ovine ovarian granulosa cells through the SREBPs/MVK-LHR pathway. Animal reproduction science, 2025 (PubMed: 39615443) [IF=2.2]

3). Estrogen deficiency exacerbates learning and memory deficits associated with glucose metabolism disorder in APP/PS1 double transgenic female mice. Genes & Diseases, 2022 (PubMed: 35873026)

Application: WB Species: Mouse Sample:

Figure 1 Estrogen levels in the circulation and brain of mice. (A) Image of uteri from all groups. (B) Quantification of uterus weight. (C) Western blot analysis of aromatase (CYP19A1) expression. β-actin was used as an internal control. (D) Densitometric analysis of CYP19A1 expression normalized to β-actin. Data are presented as the mean ± SEM. Compared with WT-Sham, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; compared with AD-Sham

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Cytochrome P450 19A1 Antibody - #DF6884