NR1H3 Antibody - #DF6864

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Product: NR1H3 Antibody
Catalog: DF6864
Description: Rabbit polyclonal antibody to NR1H3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50kDa; 50kD(Calculated).
Uniprot: Q13133
RRID: AB_2838823

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(0%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NR1H3 Antibody detects endogenous levels of total NR1H3.
RRID:
AB_2838823
Cite Format: Affinity Biosciences Cat# DF6864, RRID:AB_2838823.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Liver X receptor alpha; LXR a; LXRA; NR1H3; NR1H3_HUMAN; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR alpha; Oxysterols receptor LXR-alpha; RLD 1; RLD1;

Immunogens

Immunogen:
Uniprot:

Q13133(NR1H3_HUMAN) >>Visit HPA database.

Gene(ID):
NR1H3(10062)
Expression:
Q13133 NR1H3_HUMAN:

Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

Description:
The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Sequence:
MSLWLGAPVPDIPPDSAVELWKPGAQDASSQAQGGSSCILREEARMPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13133 As Substrate

Site PTM Type Enzyme
S198 Phosphorylation P68400 (CSNK2A1)
S246 Phosphorylation
Y306 Phosphorylation
S310 Phosphorylation
S312 Phosphorylation
T314 Phosphorylation
K328 Sumoylation
S413 Phosphorylation
K434 Sumoylation
K434 Ubiquitination

Research Backgrounds

Function:

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (By similarity). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity).

PTMs:

Ubiquitinated leading to its degradation by the proteasome.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

Subunit Structure:

Heterodimer of NR1H3 and RXR (retinoic acid receptor). Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with SIRT1 and this interaction is inhibited by CCAR2. Interacts with GPS2.

Family&Domains:

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Nobiletin Ameliorates Hepatic Lipid Deposition, Oxidative Stress, and Inflammation by Mechanisms That Involve the Nrf2/NF-κB Axis in Nonalcoholic Fatty Liver Disease. Journal of agricultural and food chemistry, 2023 (PubMed: 38073108) [IF=6.1]
2). Berberine Promotes OATP1B1 Expression and Rosuvastatin Uptake by Inducing Nuclear Translocation of FXR and LXRα. Frontiers in Pharmacology, 2020 (PubMed: 32292349) [IF=5.6]

Application: WB    Species: human    Sample: HepG2 cells

FIGURE 5 | Silencing hFXR and hLXRa expression abolished berberine-induced OATP1B1 expression in HepG2 cells. HepG2 cells were transiently transfected with small interfering RNA (siRNA)-hFXR or siRNA-hLXRa, and 48 h later protein levels of FXR (A) and LXRa (C) were examined by Western blot. HepG2 cells were then treated with berberine (25 mM), GW4064, or GW3965 (10 mM) for 24 h, OATP1B1 protein levels were analyzed by Western blot (B, D).
3). Guang Chen Pi (the pericarp of Citrus reticulata Blanco's cultivars 'Chachi') inhibits macrophage-derived foam cell formation. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 35489660) [IF=5.4]
4). PPARα Agonist Exerts Protective Effects in Podocyte Injury via Inhibition of the ANGPTL3 Pathway. EXPERIMENTAL CELL RESEARCH, 2021 (PubMed: 34499887) [IF=3.7]

Application: WB    Species: Mouse    Sample: podocytes

Fig. 1. Expression of ANGPTL3, LXR α , RXR α , and PPAR α in normal and PAN-treated mouse podocytes. (A) Representative gel image for Lxr, Rxr, Ppar α , Hnf, Pparβ, and Pparγ mRNA expression in unstimulated podocytes (MPC5). A 100 ng sample of RNA from MPC5 was reverse transcribed into single-stranded cDNA. The cDNA was used as a template for PCR. PCR products were resolved in a 1.2% agarose ethidium bromide gel and visualized with ultraviolet light. Real-time RT-PCR analysis (for mRNA expression) (B), western blotting (C), and quantification (D) of ANGPTL3, LXR α , RXR α , and PPAR α in the podocytes after treatment with PAN for 0, 6, 12, 18, and 24 h *P < 0.05, **P < 0.01 vs. 0 h in the same groups.
5). Three polymethoxyflavones from the peel of Citrus reticulata “Chachi” inhibits oxidized low-density lipoprotein-induced macrophage-derived foam cell formation. Frontiers in Cardiovascular Medicine, 2022 (PubMed: 35966555) [IF=3.6]
6). Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2. Oncology Research, 2023 (PubMed: 37305391) [IF=3.1]

Application: WB    Species: Mouse    Sample:

Figure 4 Calcyclin-binding protein (CACYBP) knockdown facilitated degradation of minichromosome maintenance complex component 2 (MCM2) by inducing ubiquitination. (A) Relative mRNA levels of the top eleven differentially expressed genes (DEGs) in HUCCT1 cells after CACYBP silencing as determined by qPCR analysis. (B) Relative protein levels of MCM2, NR1H3, PRIM1, CCND1 and CCNG1 in HUCCT1 cells after CACYBP silencing as determined by western blot assays. (C) Viability of HUCCT1 cells after knockdown of NR1H3, PRIM1, CCND1, CCNG1 and MCM2 as assessed by Celigo cell count assays at indicated times. (D) Relative protein level of MCM2 in CACYBP-knockdown HUCCT1 cells treated with cycloheximide (50 mg/mL). (E) Relative protein level of MCM2 in CACYBP-knockdown HUCCT1 cells exposed to 10 μmol/L proteasome inhibitor MG-132 treatment. (F) Ubiquitination of MCM2 immunoprecipitated by anti-MCM2 antibody. Data are represented by mean ± standard deviation. (G) Detection of K48 and K63 mediated ubiquitination of MCM2 in HUCCT1 cells. *p < 0.05, **p < 0.01, ***p < 0.001.

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