THY1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from fetal brain, using THY1 antibody.

Product:	THY1 Antibody
Catalog:	DF6849
Description:	Rabbit polyclonal antibody to THY1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Dog
Mol.Wt.:	18kDa; 18kD(Calculated).
Uniprot:	P04216
RRID:	AB_2838808

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Horse(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

THY1 Antibody detects endogenous levels of total THY1.

RRID:

AB_2838808
Cite Format: Affinity Biosciences Cat# DF6849, RRID:AB_2838808.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD7; CD90; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 T cell antigen; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein; Thy1; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; THY1_HUMAN; Thymus cell antigen 1, theta;

Immunogens

Immunogen:

Uniprot:

P04216(THY1_HUMAN) >>Visit HPA database.

Gene(ID):

THY1(7070)

Description:

Thy1 is a GPI-anchored, developmentally regulated protein involved in various signaling cascades that mediate neurite outgrowth, T cell activation, tumor suppression, apoptosis, and fibrosis (1). It is highly expressed on the surface of adult neurons and is thought to play a role in modulating adhesive and migratory events, such as neurite extension (1,2). Decreased Thy1 expression is associated with the development of epithelial ovarian cancer, revealing its role as a putative tumor suppressor gene of human ovarian cancer (3,4). Thy1 knockout mice have impaired cutaneous immune responses and abnormal retinal development (5,6). Thy1 is epigenetically regulated, or deregulated in some disease states, such as pulmonary fibrosis. The potentially reversible hypermethylation of the Thy1 promoter offers the possibility of novel therapeutic options in this disease (7).

Sequence:

P04216 THY1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNLAISIALLLTVLQVSRGQKVTSLTACLVDQSLRLDCRHENTSSSPIQYEFSLTRETKKHVLFGTVGVPEHTYRSRTNFTSKYNMKVLYLSAFTSKDEGTYTCALHHSGHSPPISSQNVTVLRDKLVKCEGISLLAQNTSWLLLLLLSLSLLQATDFMSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Horse

Rabbit

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04216 As Substrate

P04216

Site	PTM Type	Source
N42	N-Glycosylation	Uniprot
S76	Phosphorylation	Uniprot
Y90	Phosphorylation	Uniprot
T95	Phosphorylation	Uniprot
S96	Phosphorylation	Uniprot
N119	N-Glycosylation	Uniprot

Research Backgrounds

P04216_THY1_HUMAN

Function:

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Subcellular Location:

Cell membrane>Lipid-anchor.

Research Fields

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

THY1 Antibody - #DF6849