THBS1 Antibody - #DF6848
Product: | THBS1 Antibody |
Catalog: | DF6848 |
Description: | Rabbit polyclonal antibody to THBS1 |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Horse, Rabbit, Dog |
Mol.Wt.: | 129kDa; 129kD(Calculated). |
Uniprot: | P07996 |
RRID: | AB_2838807 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF6848, RRID:AB_2838807.
Fold/Unfold
THBS 1; THBS; Thbs1; Thrombospondin 1; Thrombospondin-1; TSP 1; TSP; TSP1; TSP1_HUMAN;
Immunogens
- P07996 TSP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P07996 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S56 | Phosphorylation | Uniprot | |
K173 | Ubiquitination | Uniprot | |
N248 | N-Glycosylation | Uniprot | |
K262 | Ubiquitination | Uniprot | |
T289 | O-Glycosylation | Uniprot | |
T292 | O-Glycosylation | Uniprot | |
T293 | O-Glycosylation | Uniprot | |
T302 | O-Glycosylation | Uniprot | |
K306 | Ubiquitination | Uniprot | |
Y319 | Phosphorylation | Uniprot | |
K472 | Ubiquitination | Uniprot | |
T481 | Phosphorylation | Uniprot | |
T563 | Phosphorylation | Uniprot | |
S564 | Phosphorylation | Uniprot | |
Y565 | Phosphorylation | Uniprot | |
S569 | Phosphorylation | Uniprot | |
S627 | Phosphorylation | Uniprot | |
T638 | O-Glycosylation | Uniprot | |
Y817 | Phosphorylation | Uniprot | |
Y1022 | Phosphorylation | Uniprot | |
Y1029 | Phosphorylation | Uniprot | |
S1033 | Phosphorylation | Uniprot | |
Y1036 | Phosphorylation | Uniprot | |
Y1058 | Phosphorylation | Uniprot | |
N1067 | N-Glycosylation | Uniprot |
Research Backgrounds
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).
Secreted. Cell surface. Secreted>Extracellular space>Extracellular matrix. Endoplasmic reticulum. Sarcoplasmic reticulum.
Note: Secreted by thrombin-activated platelets and binds to the cell surface in the presence of extracellular Ca(2+) (PubMed:6777381). Incorporated into the extracellular matrix of fibroblasts (PubMed:6341993). Also detected in the endoplasmic reticulum and sarcoplasmic reticulum where it plays a role in the ER stress response (By similarity).
Homotrimer; disulfide-linked. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 (By similarity). Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts with ATF6 (via lumenal domain) (By similarity).
Belongs to the thrombospondin family.
Research Fields
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Malaria.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)
References
Application: IHC Species: Human Sample: SGC tissues
Application: WB Species: Human Sample: SGC cells
Application: IF/ICC Species: Human Sample: SGC cells
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