UAP1 Antibody - #DF6843

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Product: UAP1 Antibody
Catalog: DF6843
Description: Rabbit polyclonal antibody to UAP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 57kDa; 59kD(Calculated).
Uniprot: Q16222
RRID: AB_2838802

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
UAP1 Antibody detects endogenous levels of total UAP1.
RRID:
AB_2838802
Cite Format: Affinity Biosciences Cat# DF6843, RRID:AB_2838802.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGX 1; AGX; AGX-1; AGX-2; AGX1; Antigen X; AntigenX; SPAG 2; SPAG2; Sperm associated antigen 2; Sperm-associated antigen 2; UAP 1; uap1; UAP1_HUMAN; UDP N acetylhexosamine pyrophosphorylase; UDP N acteylglucosamine pyrophosphorylase 1; UDP-N-acetylglucosamine pyrophosphorylase;

Immunogens

Immunogen:
Uniprot:

Q16222(UAP1_HUMAN) >>Visit HPA database.

Gene(ID):
UAP1(6675)
Expression:
Q16222 UAP1_HUMAN:

Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver.

Description:
UAP1 belongs to the DPGP type 1 family. It converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P (1).
Sequence:
MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSHQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIILEEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQHHVAQKKIPYVDTQGQLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTTARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRSATNGKSETITADVNHNLKDANDVPIQCEISPLISYAGEGLESYVADKEFHAPLIIDENGVHELVKNGI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16222 As Substrate

Site PTM Type Enzyme
K7 Ubiquitination
K12 Ubiquitination
K63 Ubiquitination
K122 Ubiquitination
Y125 Phosphorylation
K133 Ubiquitination
K145 Ubiquitination
K152 Ubiquitination
Y153 Phosphorylation
K157 Ubiquitination
T170 Phosphorylation
K175 Ubiquitination
K180 Acetylation
K180 Ubiquitination
K182 Acetylation
K182 Ubiquitination
K188 Ubiquitination
K213 Ubiquitination
K215 Ubiquitination
K271 Ubiquitination
K278 Ubiquitination
K282 Ubiquitination
C293 S-Nitrosylation
Y299 Phosphorylation
Y304 Phosphorylation
K314 Ubiquitination
K356 Ubiquitination
K357 Ubiquitination
Y360 Phosphorylation
T363 Phosphorylation
K369 Ubiquitination
K372 Ubiquitination
K377 Ubiquitination
K380 Ubiquitination
K390 Ubiquitination
K391 Ubiquitination
S404 Phosphorylation
K407 Ubiquitination
K415 Ubiquitination
K472 Acetylation
K472 Ubiquitination
K519 Ubiquitination

Research Backgrounds

Function:

Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.

Subcellular Location:

Cytoplasm.
Note: In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver.

Subunit Structure:

Monomer and homodimer. Isoform AGX1 is a homodimer. Isoform AGX2 is a monomer.

Family&Domains:

Belongs to the UDPGP type 1 family.

Research Fields

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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