Product: SORD Antibody
Catalog: DF6842
Description: Rabbit polyclonal antibody to SORD
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 38kDa; 38kD(Calculated).
Uniprot: Q00796
RRID: AB_2838801

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
SORD Antibody detects endogenous levels of total SORD.
RRID:
AB_2838801
Cite Format: Affinity Biosciences Cat# DF6842, RRID:AB_2838801.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHSO_HUMAN; L iditol 2 dehydrogenase; L-iditol 2-dehydrogenase; OTTHUMP00000161939; SDH; Sorbitol dehydrogenase 1; Sorbitol dehydrogenase; SORD 1; SORD; SORD1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q00796 DHSO_HUMAN:

Expressed in liver (PubMed:3365415). Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).

Description:
SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626).
Sequence:
MAAAAKPNNLSLVVHGPGDLRLENYPIPEPGPNEVLLRMHSVGICGSDVHYWEYGRIGNFIVKKPMVLGHEASGTVEKVGSSVKHLKPGDRVAIEPGAPRENDEFCKMGRYNLSPSIFFCATPPDDGNLCRFYKHNAAFCYKLPDNVTFEEGALIEPLSVGIHACRRGGVTLGHKVLVCGAGPIGMVTLLVAKAMGAAQVVVTDLSATRLSKAKEIGADLVLQISKESPQEIARKVEGQLGCKPEVTIECTGAEASIQAGIYATRSGGNLVLVGLGSEMTTVPLLHAAIREVDIKGVFRYCNTWPVAISMLASKSVNVKPLVTHRFPLEKALEAFETFKKGLGLKIMLKCDPSDQNP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
92
Dog
92
Xenopus
92
Chicken
92
Rabbit
92
Zebrafish
83
Pig
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q00796 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K6 Acetylation
K6 Ubiquitination
S11 Phosphorylation
Y25 Phosphorylation
Y51 Phosphorylation
Y54 Phosphorylation
K64 Ubiquitination
K78 Ubiquitination
K84 Ubiquitination
K107 Ubiquitination
T122 Phosphorylation
K134 Ubiquitination
R167 Methylation
S206 Phosphorylation
T208 Phosphorylation
S211 Phosphorylation
S225 Phosphorylation
K226 Ubiquitination
S228 Phosphorylation
K295 Ubiquitination
K319 Ubiquitination
K339 Acetylation
K339 Ubiquitination
K349 Ubiquitination

Research Backgrounds

Function:

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively. Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility. May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP(+) as the electron acceptor.

Subcellular Location:

Mitochondrion membrane>Peripheral membrane protein. Cell projection>Cilium>Flagellum.
Note: Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in liver. Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the zinc-containing alcohol dehydrogenase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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