ERK5 Antibody - #DF6835

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Product: ERK5 Antibody
Catalog: DF6835
Description: Rabbit polyclonal antibody to ERK5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 89kDa; 88kD(Calculated).
Uniprot: Q13164
RRID: AB_2838794

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ERK5 Antibody detects endogenous levels of total ERK5.
RRID:
AB_2838794
Cite Format: Affinity Biosciences Cat# DF6835, RRID:AB_2838794.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1; BMK1 Kinase; EC 2.7.11.24; ERK 4; ERK 5; ERK-5; ERK4; ERK5; Extracellular signal regulated kinase 5; Extracellular signal-regulated kinase 5; MAP kinase 7; MAPK 7; MAPK7; Mitogen activated protein kinase 7; Mitogen-activated protein kinase 7; MK07_HUMAN; OTTHUMP00000065906; OTTHUMP00000065907; PRKM 7; PRKM7; PROTEIN KINASE, MITOGEN-ACTIVATED, 7;

Immunogens

Immunogen:
Uniprot:

Q13164(MK07_HUMAN) >>Visit HPA database.

Gene(ID):
MAPK7(5598)
Expression:
Q13164 MK07_HUMAN:

Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.

Description:
ERK5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1,5-7). Like other MAPK family members, ERK5 contains a canonical activation loop TEY motif (Thr218/Tyr220) which is specifically phosphorylated by MAP2K5 (MEK5) in a growth factor-dependent, Ras-independent mechanism (2-4). For example, EGF stimulation promotes ERK5 phosphorylation which induces its traslocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (2,3). ERK5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoetic progenitor cells where it promotes survival and proliferation (4). In neuronal cells, ERK5 is required for NGF-induced neurite outgrowth, neuronal homeostatis, and survival (11,12). ERK5 is thought to play a role in blood vessel integrity via maintainence of endothelial cell migration and barrier function (8-10). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting ERK5 plays a critical role in vascular development and homeostasis (1,5).
Sequence:
MAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPGCPDVEMPSPWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAISDNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAPAPTPTPTPVQPTSPPPGPVAQPTGPQPQSAGSTSGPVPQPACPPPGPAPHPTGPPGPIPVPAPPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13164 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
T28 Phosphorylation Q13164 (MAPK7)
S31 Phosphorylation
K35 Acetylation
S70 Phosphorylation
S71 Phosphorylation
K110 Ubiquitination
K118 Ubiquitination
K184 Ubiquitination
Y216 Phosphorylation
T219 Phosphorylation Q13163 (MAP2K5)
Y221 Phosphorylation Q13163 (MAP2K5)
T224 Phosphorylation
T280 Phosphorylation
S282 Phosphorylation
S322 Phosphorylation
K378 Ubiquitination
S421 Phosphorylation Q13164 (MAPK7)
S433 Phosphorylation Q13164 (MAPK7)
S472 Phosphorylation
K476 Ubiquitination
K480 Methylation
K485 Methylation
S486 Phosphorylation Q05513 (PRKCZ)
S496 Phosphorylation Q13164 (MAPK7) , Q15418 (RPS6KA1)
S562 Phosphorylation
S567 Phosphorylation
T584 Phosphorylation
S707 Phosphorylation
S718 Phosphorylation
S720 Phosphorylation
S731 Phosphorylation Q13164 (MAPK7)
T733 Phosphorylation Q13164 (MAPK7) , P27361 (MAPK3) , P28482 (MAPK1)
S754 Phosphorylation
S770 Phosphorylation Q13164 (MAPK7)
S772 Phosphorylation Q13164 (MAPK7)
S774 Phosphorylation Q13164 (MAPK7)
S776 Phosphorylation
S803 Phosphorylation

PTMs - Q13164 As Enzyme

Substrate Site Source
O00141-1 (SGK1) S78 Uniprot
P01100 (FOS) S32 Uniprot
P01100 (FOS) T232 Uniprot
P14921-1 (ETS1) T38 Uniprot
P17302 (GJA1) S255 Uniprot
P17302 (GJA1) S282 Uniprot
P19793-1 (RXRA) T82 Uniprot
P19793-1 (RXRA) S260 Uniprot
P29590 (PML) S403 Uniprot
P29590 (PML) T409 Uniprot
Q02078-5 (MEF2A) T304 Uniprot
Q02078-5 (MEF2A) T311 Uniprot
Q02078 (MEF2A) T312 Uniprot
Q02078 (MEF2A) T319 Uniprot
Q02078-5 (MEF2A) S347 Uniprot
Q02078 (MEF2A) S355 Uniprot
Q06413-3 (MEF2C) S387 Uniprot
Q12959 (DLG1) S158 Uniprot
Q13163 (MAP2K5) S129 Uniprot
Q13163 (MAP2K5) S137 Uniprot
Q13163 (MAP2K5) S142 Uniprot
Q13163 (MAP2K5) S149 Uniprot
Q13164 (MAPK7) T28 Uniprot
Q13164 (MAPK7) S421 Uniprot
Q13164 (MAPK7) S433 Uniprot
Q13164 (MAPK7) S496 Uniprot
Q13164 (MAPK7) S731 Uniprot
Q13164 (MAPK7) T733 Uniprot
Q13164 (MAPK7) S770 Uniprot
Q13164 (MAPK7) S772 Uniprot
Q13164 (MAPK7) S774 Uniprot
Q14814-1 (MEF2D) S180 Uniprot
Q14934 (NFATC4) S168 Uniprot
Q14934 (NFATC4) S170 Uniprot
Q92886 (NEUROG1) S178 Uniprot
Q92886 (NEUROG1) S201 Uniprot

Research Backgrounds

Function:

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.

PTMs:

Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus>PML body.
Note: Translocates to the nucleus upon activation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.

Subunit Structure:

Interacts with MAP2K5. Forms oligomers (By similarity). Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D (By similarity). Interacts with SGK1. Preferentially interacts with PML isoform PML-4 but shows interaction also with its other isoforms: isoform PML-1, isoform PML-2, isoform PML-3 and isoform PML-6. Interacts (via N-terminal half) with HSP90AB1-CDC37 chaperone complex in resting cells; the interaction is MAP2K5-independent and prevents MAPK7 from ubiquitination and proteasomal degradation.

Family&Domains:

The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). Combining Bioinformatics and Experiments to Identify MAD2L2 as a Key Regulator in Ovarian Cancer. Research Square, 2022

Application: WB    Species: Human    Sample:

Figure 9. The expression of MAD2L2 and mTOR signaling. (A). The western blot results of MAD2L2 and mTOR signaling relative proteins. (B. C. D). The predictive correlation of mTOR and ferroptosis relative genes. (E). The western blot results of mTOR and ferroptosis relative proteins.

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