PPA1 Antibody - #DF6832
(3)
| Product: | PPA1 Antibody |
| Catalog: | DF6832 |
| Description: | Rabbit polyclonal antibody to PPA1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 33kDa; 33kD(Calculated). |
| Uniprot: | Q15181 |
| RRID: | AB_2838791 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
PPA1 Antibody detects endogenous levels of total PPA1.
RRID:
AB_2838791
Cite Format: Affinity Biosciences Cat# DF6832, RRID:AB_2838791.
Cite Format: Affinity Biosciences Cat# DF6832, RRID:AB_2838791.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
cytosolic inorganic pyrophosphatase; diphosphate phosphohydrolase; inorganic diphosphatase; inorganic pyrophosphatase 1; Inorganic pyrophosphatase; IOPPP; IPYR_HUMAN; PP; PP1; PPA1; PPase; Pyp; pyrophosphatase, inorganic, 1; Pyrophosphate phospho hydrolase; Pyrophosphate phospho-hydrolase; SID6-8061;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Description:
PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).This antibody is specific to PPA1.
Sequence:
- Q15181 IPYR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKVLGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEFKDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDVDKWFHHQKN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Pig
92
Horse
92
Dog
92
Chicken
91
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15181 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| T6 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| Y38 | Phosphorylation | Uniprot | |
| K57 | Acetylation | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K70 | Acetylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K74 | Acetylation | Uniprot | |
| K75 | Acetylation | Uniprot | |
| Y80 | Phosphorylation | Uniprot | |
| Y94 | Phosphorylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| R132 | Methylation | Uniprot | |
| K155 | Acetylation | Uniprot | |
| K176 | Acetylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| Y193 | Phosphorylation | Uniprot | |
| K194 | Acetylation | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K228 | Acetylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| T246 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| C270 | S-Nitrosylation | Uniprot | |
| C274 | S-Nitrosylation | Uniprot | |
| K282 | Ubiquitination | Uniprot |
Research Backgrounds
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed ubiquitously.
Subunit Structure:
Homodimer.
Family&Domains:
Belongs to the PPase family.
Research Fields
· Metabolism > Energy metabolism > Oxidative phosphorylation.
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