p40 phox Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from HUVEC, using NCF4 Antibody.

DF6820 at 1/100 staining Mouse brain tissue by IHC-P.

Product:	p40 phox Antibody
Catalog:	DF6820
Description:	Rabbit polyclonal antibody to p40 phox
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	39kDa; 39kD(Calculated).
Uniprot:	Q15080
RRID:	AB_2838780

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(83%), Rabbit(100%), Dog(83%)

Clonality:

Polyclonal

Specificity:

p40 phox Antibody detects endogenous levels of total p40 phox.

RRID:

AB_2838780
Cite Format: Affinity Biosciences Cat# DF6820, RRID:AB_2838780.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CGD3; MGC3810; NCF 4; NCF; NCF-4; Ncf4; NCF4_HUMAN; Neutrophil cytosol factor 4; Neutrophil cytosolic factor 4; Neutrophil NADPH oxidase factor 4; p40-phox; p40phox; SH3 and PX domain-containing protein 4; SH3PXD4;

Immunogens

Immunogen:

Uniprot:

Q15080(NCF4_HUMAN) >>Visit HPA database.

Gene(ID):

NCF4(4689)

Expression:

Q15080 NCF4_HUMAN:

Expression is restricted to hematopoietic cells.

Description:

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Sequence:

Q15080 NCF4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15080 As Substrate

Q15080

Site	PTM Type	Enzyme	Source
S11	Phosphorylation		Uniprot
S23	Phosphorylation		Uniprot
T47	Phosphorylation		Uniprot
S51	Phosphorylation		Uniprot
Y53	Phosphorylation		Uniprot
T154	Phosphorylation	Q05655 (PRKCD) , P17252 (PRKCA)	Uniprot
S161	Phosphorylation		Uniprot
S166	Phosphorylation		Uniprot
K228	Ubiquitination		Uniprot
Y243	Phosphorylation		Uniprot
Y245	Phosphorylation		Uniprot
S299	Phosphorylation		Uniprot
S315	Phosphorylation	P17252 (PRKCA)	Uniprot

Research Backgrounds

Q15080_NCF4_HUMAN

Function:

Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.

Subcellular Location:

Cytoplasm>Cytosol. Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Peripheral membrane protein.

Tissue Specificity:

Expression is restricted to hematopoietic cells.

Subunit Structure:

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. NCF4 interacts primarily with NCF2 to form a complex with NCF1.

Family&Domains:

The PB1 domain mediates the association with NCF2/p67-PHOX.

The PX domain mediates interaction with membranes enriched in phosphatidylnositol 3-phosphate.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

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p40 phox Antibody - #DF6820