GNA15 Antibody - #DF6805

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Product: GNA15 Antibody
Catalog: DF6805
Description: Rabbit polyclonal antibody to GNA15
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 44kDa, 55 kDa; 44kD(Calculated).
Uniprot: P30679
RRID: AB_2838766

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
GNA15 Antibody detects endogenous levels of total GNA15.
RRID:
AB_2838766
Cite Format: Affinity Biosciences Cat# DF6805, RRID:AB_2838766.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G alpha 15; G alpha 16; G alpha-15; G alpha-16; G-protein subunit alpha-15; G-protein subunit alpha-16; GNA 15; GNA 16; GNA15; GNA15_HUMAN; GNA16; Gq class; Guanine nucleotide binding protein alpha 15; Guanine nucleotide binding protein alpha 15 subunit; Guanine nucleotide-binding protein subunit alpha-15; Guanine nucleotide-binding protein subunit alpha-16;

Immunogens

Immunogen:
Uniprot:

P30679(GNA15_HUMAN) >>Visit HPA database.

Gene(ID):
GNA15(2769)
Expression:
P30679 GNA15_HUMAN:

Specifically expressed in hematopoietic cells. Expressed in epididymis (at protein level).

Description:
Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein , ∫ and polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).
Sequence:
MARSLTWRCCPWCLTEDEKAAARVDQEINRILLEQKKQDRGELKLLLLGPGESGKSTFIKQMRIIHGAGYSEEERKGFRPLVYQNIFVSMRAMIEAMERLQIPFSRPESKHHASLVMSQDPYKVTTFEKRYAAAMQWLWRDAGIRAYYERRREFHLLDSAVYYLSHLERITEEGYVPTAQDVLRSRMPTTGINEYCFSVQKTNLRIVDVGGQKSERKKWIHCFENVIALIYLASLSEYDQCLEENNQENRMKESLALFGTILELPWFKSTSVILFLNKTDILEEKIPTSHLATYFPSFQGPKQDAEAAKRFILDMYTRMYTGCVDGPEGSKKGARSRRLFSHYTCATDTQNIRKVFKDVRDSVLARYLDEINLL

PTMs - P30679 As Substrate

Site PTM Type Enzyme
K19 Ubiquitination
K36 Ubiquitination
K44 Ubiquitination
K55 Ubiquitination
S71 Phosphorylation
K123 Ubiquitination
R186 Methylation
K201 Ubiquitination
R205 Methylation
K213 Ubiquitination
S214 Phosphorylation
K285 Ubiquitination
K302 Ubiquitination
Y316 Phosphorylation
S330 Phosphorylation
S336 Phosphorylation
K354 Ubiquitination

Research Backgrounds

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Tissue Specificity:

Specifically expressed in hematopoietic cells. Expressed in epididymis (at protein level).

Subunit Structure:

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Family&Domains:

Belongs to the G-alpha family. G(q) subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

Restrictive clause

 

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For Research Use Only.
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