EPHX1 Antibody - #DF6799

(3)  
Product: EPHX1 Antibody
Catalog: DF6799
Description: Rabbit polyclonal antibody to EPHX1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 53kDa; 53kD(Calculated).
Uniprot: P07099
RRID: AB_2838761

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(90%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(90%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
EPHX1 Antibody detects endogenous levels of total EPHX1.
RRID:
AB_2838761
Cite Format: Affinity Biosciences Cat# DF6799, RRID:AB_2838761.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EPHX 1; EPHX; Ephx1; EPOX; Epoxide hydratase; Epoxide hydrolase 1; Epoxide hydrolase 1 microsomal (xenobiotic); Epoxide hydroxylase 1; Epoxide hydroxylase 1 microsomal (xenobiotic); HYEP_HUMAN; HYL1; MEH; Microsomal epoxide hydrolase;

Immunogens

Immunogen:
Uniprot:

P07099(HYEP_HUMAN) >>Visit HPA database.

Gene(ID):
EPHX1(2052)
Expression:
P07099 HYEP_HUMAN:

Found in liver.

Description:
Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008]
Sequence:
MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRSAAREDDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFDWKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTERDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTNTEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKVYVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLSVLERQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Zebrafish
100
Chicken
100
Bovine
90
Dog
90
Xenopus
90
Rabbit
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07099 As Substrate

Site PTM Type Enzyme
S47 Phosphorylation
K52 Ubiquitination
T55 Phosphorylation
S56 Phosphorylation
K91 Ubiquitination
K92 Ubiquitination
S95 Phosphorylation
K105 Ubiquitination
K117 Methylation
K119 Methylation
K131 Acetylation
K168 Ubiquitination
S173 Phosphorylation
K196 Ubiquitination
K197 Ubiquitination
T204 Phosphorylation
K210 Acetylation
Y283 Phosphorylation
K286 Ubiquitination
K288 Ubiquitination
Y291 Phosphorylation
S297 Phosphorylation
Y299 Phosphorylation
Y339 Phosphorylation
Y361 Phosphorylation
K386 Acetylation
K386 Ubiquitination
Y393 Phosphorylation
T408 Phosphorylation
S422 Phosphorylation
Y423 Phosphorylation
Y425 Phosphorylation
S450 Phosphorylation

Research Backgrounds

Function:

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water (By similarity). May play a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids.

Subcellular Location:

Microsome membrane>Single-pass type III membrane protein. Endoplasmic reticulum membrane>Single-pass type III membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in liver.

Family&Domains:

Belongs to the peptidase S33 family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

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