CAPZA2 Antibody - #DF6784

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Product: CAPZA2 Antibody
Catalog: DF6784
Description: Rabbit polyclonal antibody to CAPZA2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 33kDa; 33kD(Calculated).
Uniprot: P47755
RRID: AB_2838746

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
CAPZA2 Antibody detects endogenous levels of total CAPZA2.
RRID:
AB_2838746
Cite Format: Affinity Biosciences Cat# DF6784, RRID:AB_2838746.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAPPA2; Capping protein (actin filament) muscle Z line, alpha 2; CapZ alpha 2; CapZ alpha-2; CAPZ; CAPZA2; CAZA2_HUMAN; F actin capping protein alpha 2 subunit; F actin capping protein subunit alpha 2; F-actin-capping protein subunit alpha-2;

Immunogens

Immunogen:
Uniprot:

P47755(CAZA2_HUMAN) >>Visit HPA database.

Gene(ID):
CAPZA2(830)
Description:
The protein encoded by this gene is a member of the F-actin capping protein alpha subunit family. It is the alpha subunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulates the growth of the actin filaments at the barbed end. [provided by RefSeq, Jul 2008]
Sequence:
MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P47755 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S9 Phosphorylation
K13 Ubiquitination
K19 Acetylation
K19 Ubiquitination
T63 Phosphorylation
K86 Acetylation
K86 Ubiquitination
K91 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K103 Ubiquitination
K133 Ubiquitination
T142 Phosphorylation
Y144 Phosphorylation
K146 Ubiquitination
T180 Phosphorylation
Y198 Phosphorylation
K226 Ubiquitination
K230 Ubiquitination
K256 Methylation
K256 Ubiquitination
K268 Sumoylation
K268 Ubiquitination
K273 Acetylation
K273 Sumoylation
K273 Ubiquitination
Y277 Phosphorylation
K278 Acetylation
K278 Sumoylation
K278 Ubiquitination
K281 Ubiquitination

Research Backgrounds

Function:

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASHC1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), WASHC2 (WASHC2A or WASHC2C), WASHC3, WASHC4 and WASHC5. Interacts with RCSD1/CAPZIP. Directly interacts with CRACD; this interaction decreases binding to actin.

Family&Domains:

Belongs to the F-actin-capping protein alpha subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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