cdc45 Antibody - #DF6779

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Product: cdc45 Antibody
Catalog: DF6779
Description: Rabbit polyclonal antibody to cdc45
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 64kDa; 66kD(Calculated).
Uniprot: O75419
RRID: AB_2838741

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
cdc45 Antibody detects endogenous levels of total cdc45.
RRID:
AB_2838741
Cite Format: Affinity Biosciences Cat# DF6779, RRID:AB_2838741.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC 45; CDC 45L; CDC45 (cell division cycle 45, S.cerevisiae homolog) like; CDC45; CDC45 cell division cycle 45 like; CDC45 like; CDC45 related protein; CDC45_HUMAN; CDC45L2; Cell division control protein 45 homolog; Cell division cycle 45 like 2; Cell division cycle 45 like; PORC PI 1; PORC-PI-1;

Immunogens

Immunogen:
Uniprot:

O75419(CDC45_HUMAN) >>Visit HPA database.

Gene(ID):
CDC45(8318)
Expression:
O75419 CDC45_HUMAN:

Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.

Description:
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
Sequence:
MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELETAFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLIKQDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRLEEEIVEQTMRRRQRREWEARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
92
Horse
92
Bovine
92
Sheep
92
Chicken
83
Xenopus
75
Zebrafish
67
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75419 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
S16 Phosphorylation
Y130 Phosphorylation
S144 Phosphorylation
S148 Phosphorylation
S151 Phosphorylation
T168 Phosphorylation
K237 Ubiquitination
T262 Phosphorylation
K301 Ubiquitination
K309 Ubiquitination
K323 Ubiquitination
K326 Ubiquitination
K328 Ubiquitination
K337 Ubiquitination
K350 Ubiquitination
K354 Ubiquitination
T360 Phosphorylation
K411 Ubiquitination
Y413 Phosphorylation
K420 Ubiquitination
K471 Ubiquitination
K475 Ubiquitination
K482 Ubiquitination
T513 Phosphorylation
R524 Methylation
K528 Sumoylation
K528 Ubiquitination
K550 Ubiquitination

Research Backgrounds

Function:

Required for initiation of chromosomal DNA replication.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.

Subunit Structure:

Associated with ORC2. Interacts with HELB.

Family&Domains:

Belongs to the CDC45 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

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