WDR5 Antibody - #DF6759
| Product: | WDR5 Antibody |
| Catalog: | DF6759 |
| Description: | Rabbit polyclonal antibody to WDR5 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Dog |
| Mol.Wt.: | 36kDa, 44 kDa; 37kD(Calculated). |
| Uniprot: | P61964 |
| RRID: | AB_2838721 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF6759, RRID:AB_2838721.
Fold/Unfold
2410008O07Rik; AA408785; AA960360; BIG 3; Big; BIG3; BMP2 induced 3 kb gene protein; BMP2 induced gene, 3-KB; BMP2-induced 3-kb gene protein; MGC114572; OTTHUMP00000162494; SWD 3; SWD3; SWD3 Set1c WD40 repeat protein homolog; WD repeat containing protein 5; WD repeat domain 5; WD repeat protein 5; WD repeat-containing protein 5; WD repeat-containing protein BIG-3; WDR 5; Wdr5; WDR5_HUMAN;
Immunogens
- P61964 WDR5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P61964 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T18 | Phosphorylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K38 | Methylation | Uniprot | |
| K46 | Sumoylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K112 | Acetylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| Y228 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot |
Research Backgrounds
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
Nucleus.
Interacts with PAXBP1; the interaction is direct and links a WDR5-containing histone methyltransferase complex to PAX7 and PAX3. Interacts with HCFC1. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7. Each complex is at least composed of ASH2L, RBBP5, WDR5, DPY30, one or more specific histone methyltransferases (KMT2A/MLL1, KMT2D/MLL2, KMT2C/MLL3 and KMT2B/MLL4), and the facultative components PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9, TEX10 and alpha- and beta-tubulin. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts with KMT2A/MLL1 (via WIN motif) and RBBP5; the interaction is direct. Component ofthe ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. In the complex, it probably interacts directly with KAT2A, MBIP and KAT14. Interacts with histone H3. Interacts with SETD1A (via WIN motif). Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity. Interacts with PER1 (By similarity). Interacts with KMT2B (via WIN motif), KMT2C (via WIN motif), KMT2D (via WIN motif) and SETD1B (via WIN motif).
Belongs to the WD repeat WDR5/wds family.
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