Product: RPS19 Antibody
Catalog: DF6756
Description: Rabbit polyclonal antibody to RPS19
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 16kDa; 16kD(Calculated).
Uniprot: P39019
RRID: AB_2838718

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RPS19 Antibody detects endogenous levels of total RPS19.
RRID:
AB_2838718
Cite Format: Affinity Biosciences Cat# DF6756, RRID:AB_2838718.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S19; DBA; DBA1; HGNC:10402; Ribosomal protein S19; rps19; RS19_HUMAN; S19;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P39019 RS19_HUMAN:

Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.

Description:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Sequence:
MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYGGRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDGGRKLTPQGQRDLDRIAGQVAAANKKH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P39019 As Substrate

Site PTM Type Enzyme
K7 Ubiquitination
K23 Acetylation
K29 Ubiquitination
K38 Ubiquitination
K43 Ubiquitination
Y48 Phosphorylation
Y54 Phosphorylation
T55 Phosphorylation
R56 Methylation
S59 Phosphorylation P17612 (PRKACA) , Q14012 (CAMK1)
R67 Methylation
S74 Phosphorylation
T76 Phosphorylation
K77 Acetylation
K77 Ubiquitination
R94 Methylation
K111 Acetylation
K111 Ubiquitination
K115 Acetylation
K122 Ubiquitination
R133 Methylation
K143 Acetylation
K143 Ubiquitination
K144 Acetylation

Research Backgrounds

Function:

Required for pre-rRNA processing and maturation of 40S ribosomal subunits.

Subcellular Location:

Nucleus.
Note: Located more specifically in the nucleoli.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.

Subunit Structure:

Interacts with RPS19BP1.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS19 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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