MT2A Antibody - #DF6755

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Product: MT2A Antibody
Catalog: DF6755
Description: Rabbit polyclonal antibody to MT2A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 7kDa; 6kD(Calculated).
Uniprot: P02795
RRID: AB_2838717

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MT2A Antibody detects endogenous levels of total MT2A.
RRID:
AB_2838717
Cite Format: Affinity Biosciences Cat# DF6755, RRID:AB_2838717.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CES 1; CES1; Metallothionein 1A; Metallothionein 1S; Metallothionein 2; Metallothionein 2A; Metallothionein IA; Metallothionein II; Metallothionein-1A; Metallothionein-IA; Metallothionein2; MGC32848; MT 1A; MT 2; MT 2A; MT IA; MT II; MT-1A; MT-IA; MT1; MT1A; MT1A_HUMAN; MT1S; MT2; MT2A; MTC;

Immunogens

Immunogen:
Uniprot:

P02795(MT2_HUMAN) >>Visit HPA database.

Gene(ID):
MT2A(4502)
Sequence:
MDPNCSCAAGDSCTCAGSCKCKECKCTSCKKSCCSCCPVGCAKCAQGCICKGASDKCSCCA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
57
Horse
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02795 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S6 Phosphorylation
S12 Phosphorylation
T14 Phosphorylation
S18 Phosphorylation
K22 Acetylation
S32 Phosphorylation
S35 Phosphorylation
K43 Ubiquitination
K51 Acetylation
K51 Ubiquitination
K56 Ubiquitination
S58 Phosphorylation

Research Backgrounds

Function:

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Family&Domains:

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Belongs to the metallothionein superfamily. Type 1 family.

Research Fields

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Effects of Long-Term Dietary Zinc Oxide Nanoparticle on Liver Function, Deposition, and Absorption of Trace Minerals in Intrauterine Growth Retardation Pigs. BIOLOGICAL TRACE ELEMENT RESEARCH (PubMed: 36585599) [IF=3.9]
2). Biotransformation of Graphene Oxide in Lung Fluids Enhances its Synergistic Cytotoxic Effect with Cadmium.

Application: WB    Species: Human    Sample: BEAS-2B cells

Figure 3. The cytotoxicity of Cd and intracellular Cd accumulation with or without GO pre-treatment. (a) The cellular viability of BEAS-2B cells detected by CCK8 assay after being treated to Cd (1, 5, 10, 20, 25, 30 and 50 μM) for 24 h (n = 5, * indicate P < 0.05, compared to the untreated group). (b) The heat map of the cell viability inspected by CCK8 assay for BEAS-2B cells after being treated to Cd (1, 5, 10, 20, 25, 30 and 50 μM) for 24 h with or without pre-treatment of P-GO, A-GO, or G-GO at the dose of 10 μg/mL, respectively (n = 5). (c) Intracellular Cd mass quantification by ICP-MS. BEAS-2B cells were pre-treated with P-GO, A-GO, G-GO at 10 µg/mL for 24 h, and then exposed to CdCl2 at 10 μM for 24 h (n = 5), *P < 0.05. (d) Western blot analysis of MT1M and MT2A protein expression levels in BEAS-2B cells exposed to either GOs (10 μg/mL) or Cd (10 μM) or a combination of both (GOs + Cd) for 24 h.

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