EIF5A1 Antibody - #DF6754

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Product: EIF5A1 Antibody
Catalog: DF6754
Description: Rabbit polyclonal antibody to EIF5A1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 16kDa; 17kD(Calculated).
Uniprot: P63241
RRID: AB_2838716

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 100ul $280 In stock
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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
EIF5A Antibody detects endogenous levels of total EIF5A1.
RRID:
AB_2838716
Cite Format: Affinity Biosciences Cat# DF6754, RRID:AB_2838716.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

eIF 4D; eIF 5A 1; EIF 5A; eIF 5A1; eIF-4D; eIF-5A; eIF-5A-1; eIF-5A1; eIF4D; eif5a; EIF5A1; eIF5AI; Eukaryotic initiation factor 5A; Eukaryotic initiation factor 5A isoform 1; Eukaryotic translation initiation factor 5A 1; Eukaryotic translation initiation factor 5A; Eukaryotic translation initiation factor 5A-1; IF5A1_HUMAN; MGC104255; MGC99547; Rev binding factor; Rev-binding factor; uORF A; uORF;

Immunogens

Immunogen:
Uniprot:

P63241(IF5A1_HUMAN) >>Visit HPA database.

Gene(ID):
EIF5A(1984)
Expression:
P63241 IF5A1_HUMAN:

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).

Description:
mRNA-binding protein involved in translation elongation. Has an important function at the level of Mrna turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
Sequence:
MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
80
Chicken
80
Xenopus
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P63241 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S15 Phosphorylation
T17 Phosphorylation
S23 Phosphorylation
K27 Acetylation
K27 Sumoylation
K27 Ubiquitination
K34 Acetylation
K34 Sumoylation
K34 Ubiquitination
K39 Acetylation
K39 Sumoylation
K39 Ubiquitination
S44 Phosphorylation
S46 Phosphorylation
K47 Acetylation
K47 Ubiquitination
K55 Acetylation
T65 Phosphorylation
K67 Acetylation
K67 Sumoylation
K67 Ubiquitination
K68 Acetylation
K68 Methylation
K68 Sumoylation
K68 Ubiquitination
Y69 Phosphorylation
K85 Sumoylation
K85 Ubiquitination
Y98 Phosphorylation
S100 Phosphorylation
R113 Methylation
K121 Acetylation
K121 Ubiquitination
K126 Sumoylation
K126 Ubiquitination
Y127 Phosphorylation
K150 Ubiquitination

Research Backgrounds

Function:

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

PTMs:

Acetylated. Deacetylated by SIRT2.

eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Subcellular Location:

Cytoplasm. Nucleus. Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Nucleus>Nuclear pore complex.
Note: Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).

Subunit Structure:

Interacts with DHPS, with SDCBP and DOHH. Interacts with HIV-1 protein Rev. Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.

Family&Domains:

Belongs to the eIF-5A family.

References

1). Comparison of the major cell populations among osteoarthritis, Kashin–Beck disease and healthy chondrocytes by single-cell RNA-seq analysis. Cell Death & Disease, 2021 (PubMed: 34045450) [IF=8.1]

Application: IHC    Species: Human    Sample: healthy cartilage tissues

Fig. 1 Single-cell RNA-sequencing analysis of human healthy control chondrocyte tissue samples. A Seven healthy human chondrocyte clusters. Visualisation of clustering by t-SNE plot of healthy control samples, identified by cell type. B Monocle pseudotime trajectory showing the progression of seven clusters of healthy chondrocytes. C Heat map of scaled gene expression data for the top 10 differentially expressed genes identifying each cluster, with selected genes listed. D Gene expression plots demonstrating high expression of SH3BGRL3 in SH3BGRL3hi fibrocartilage chondrocytes-1, IGFBP5 in IGFBP5hi fibrocartilage chondrocytes-2, CDC20 in CDC20hi cartilage progenitor cells-1, KIAA0101 in KIAA0101hi cartilage progenitor cells-2, EIF5A in EIF5Ahi regulatory chondrocytes, SOX9 in SOX9hi prehypertrophic chondrocytes and TXNIP in TXNIPhi homeostatic chondrocytes. Dot colour corresponds to the level of gene expression in each cell. t-SNE, t-distributed stochastic neighbour embedding. E Representative immunohistochemistry staining of SH3BGRL3, IGFBP5, BIRC5, SOX9, EIF5A and CDC20 in healthy cartilage tissues. Scale bar, left, 500 μm; right, 50 μm, and quantification of positive cells of different areas (superficial, middle, deep) in cartilage tissues displayed by box plot (n = 5). *p < 0.05.

Application: IHC    Species: human    Sample: cartilage

Fig. 1 |Single-cell RNA-sequencing analysis of human healthy control chondrocyte tissue samples.E Representative immunohistochemistry staining of SH3BGRL3, IGFBP5, BIRC5, SOX9, EIF5A and CDC20 in healthy cartilage tissues. Scale bar, left, 500 μm; right, 50 μm, and quantification of positive cells of different areas (superficial, middle, deep) in cartilage tissues displayed by box plot (n = 5). *p < 0.05.

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