BMPR1B Antibody | Affinity Biosciences

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$Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17β-HSD3 mRNA. (D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 μM, while the concentration of 3858858 (positive control) was 0.25 μM. (E) T produced from the microsomal fraction of rat testes containing 17β-HSD3. The data are reported as the mean ± sd (n = 3) of results obtained from individual experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 are considered to be significant, vs. the control.$

Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells.

FIGURE 4 | Downregulation of lncRNA SNHG6 regulates miR-125b-5p/BMPR1B signaling pathway in TNBC cells.

Product:	BMPR1B Antibody
Catalog:	DF6748
Description:	Rabbit polyclonal antibody to BMPR1B
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.:	57kDa; 57kD(Calculated).
Uniprot:	O00238
RRID:	AB_2838710

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

BMPR1B Antibody detects endogenous levels of total BMPR1B.

RRID:

AB_2838710
Cite Format: Affinity Biosciences Cat# DF6748, RRID:AB_2838710.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activin receptor like kinase 6; Acvrlk6; ALK 6; ALK6; alk6tr; BMP type-1B receptor; BMPR IB; BMPR-1B; Bmpr1b; BMPRIB; BMR1B_HUMAN; Bone morphogenetic protein receptor type 1B; Bone morphogenetic protein receptor type IB; Bone morphogenetic protein receptor type-1B; BR 1b; BR1b; CDw 293; CDw293; CDw293 antigen; CFK 43a; CFK43a; Serine/threonine receptor kinase; zALK 6; zALK6;

Immunogens

Immunogen:

Uniprot:

O00238(BMR1B_HUMAN) >>Visit HPA database.

Gene(ID):

BMPR1B(658)

Description:

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Sequence:

O00238 BMR1B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLRSAGKLNVGTKKEDGESTAPTPRPKVLRCKCHHHCPEDSVNNICSTDGYCFTMIEEDDSGLPVVTSGCLGLEGSDFQCRDTPIPHQRRSIECCTERNECNKDLHPTLPPLKNRDFVDGPIHHRALLISVTVCSLLLVLIILFCYFRYKRQETRPRYSIGLEQDETYIPPGESLRDLIEQSQSSGSGSGLPLLVQRTIAKQIQMVKQIGKGRYGEVWMGKWRGEKVAVKVFFTTEEASWFRETEIYQTVLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDYLKSTTLDAKSMLKLAYSSVSGLCHLHTEIFSTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVKFISDTNEVDIPPNTRVGTKRYMPPEVLDESLNRNHFQSYIMADMYSFGLILWEVARRCVSGGIVEEYQLPYHDLVPSDPSYEDMREIVCIKKLRPSFPNRWSSDECLRQMGKLMTECWAHNPASRLTALRVKKTLAKMSESQDIKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Chicken

100

Rabbit

100

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00238 As Substrate

O00238

Site	PTM Type	Source
	Phosphorylation	Uniprot
S92	Phosphorylation	Uniprot
T97	Phosphorylation	Uniprot
T109	O-Glycosylation	Uniprot
S175	Phosphorylation	Uniprot
Y290	Phosphorylation	Uniprot
S300	Phosphorylation	Uniprot
K336	Ubiquitination	Uniprot
S402	Phosphorylation	Uniprot
K493	Ubiquitination	Uniprot

PTMs - O00238 As Enzyme

O00238

Substrate	Site	Source
O95630-1 (STAMBP)	S2	Uniprot
O95630-1 (STAMBP)	S48	Uniprot
O95630-1 (STAMBP)	S243	Uniprot
O95630-1 (STAMBP)	S245	Uniprot
O95630-1 (STAMBP)	S247	Uniprot
Q15796 (SMAD2)	S464	Uniprot
Q15796 (SMAD2)	S465	Uniprot
Q15796 (SMAD2)	S467	Uniprot
Q15797 (SMAD1)	S462	Uniprot
Q15797 (SMAD1)	S463	Uniprot
Q15797 (SMAD1)	S465	Uniprot

Research Backgrounds

O00238_BMR1B_HUMAN

Function:

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. Positively regulates chondrocyte differentiation through GDF5 interaction.

Subcellular Location:

Cell membrane. Membrane>Single-pass type I membrane protein.

Subunit Structure:

Interacts with high affinity with GDF5; positively regulates chondrocyte differentiation.

Family&Domains:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

References

1). LncRNA SNHG6/miR-125b-5p/BMPR1B Axis: A New Therapeutic Target for Triple-Negative Breast Cancer. Frontiers in Oncology, 2021 (PubMed: 34026654) [IF=4.7]

Application: WB Species: Human Sample: LC540 cells

Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17β-HSD3 mRNA. (D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 μM, while the concentration of 3858858 (positive control) was 0.25 μM. (E) T produced from the microsomal fraction of rat testes containing 17β-HSD3. The data are reported as the mean ± sd (n = 3) of results obtained from individual experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 are considered to be significant, vs. the control.

Application: WB Species: Sample: TNBC cells

FIGURE 4 | Downregulation of lncRNA SNHG6 regulates miR-125b-5p/BMPR1B signaling pathway in TNBC cells.(G) miR-125b-5p inhibition upregulated BMPR1B protein expression.

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BMPR1B Antibody - #DF6748