S100A10 Antibody | Affinity Biosciences

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Western blot analysis of SW480 cell lysates, using S100A10 Antibody.

Product:	S100A10 Antibody
Catalog:	DF6738
Description:	Rabbit polyclonal antibody to S100A10
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	11kDa; 11kD(Calculated).
Uniprot:	P60903
RRID:	AB_2838700

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

S100A10 Antibody detects endogenous levels of total S100A10.

RRID:

AB_2838700
Cite Format: Affinity Biosciences Cat# DF6738, RRID:AB_2838700.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

42C; AA409961; AL024248; Annexin II ligand; Annexin II ligand, calpactin I, light polypeptide; Annexin II tetramer (AIIt) p11 subunit; Annexin II, light chain; ANX2L; ANX2LG; Ca[1]; CAL12; CAL1L; Calpactin I light chain; Calpactin I, p11 subunit; Calpactin-1 light chain; Cellular ligand of annexin II; CLP11; GP11; MGC111133; Nerve growth factor-induced protein 42C; OTTHUMP00000015269; OTTHUMP00000015270; p10; p10 protein; p11; Protein S100 A10; Protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)); S100 calcium binding protein A10 (calpactin); S100 calcium binding protein A10; S100 calcium-binding protein A10; S100a10; S10AA_HUMAN;

Immunogens

Immunogen:

Uniprot:

P60903(S10AA_HUMAN) >>Visit HPA database.

Gene(ID):

S100A10(6281)

Description:

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.

Sequence:

P60903 S10AA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPSQMEHAMETMMFTFHKFAGDKGYLTKEDLRVLMEKEFPGFLENQKDPLAVDKIMKDLDQCRDGKVGFQSFFSLIAGLTIACNDYFVVHMKQKGKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60903 As Substrate

P60903

Site	PTM Type	Source
S3	Phosphorylation	Uniprot
T11	Phosphorylation	Uniprot
K18	Ubiquitination	Uniprot
K23	Acetylation	Uniprot
K23	Ubiquitination	Uniprot
Y25	Phosphorylation	Uniprot
T27	Phosphorylation	Uniprot
K28	Acetylation	Uniprot
K28	Ubiquitination	Uniprot
K37	Acetylation	Uniprot
K37	Ubiquitination	Uniprot
K47	Ubiquitination	Uniprot
K54	Acetylation	Uniprot
K54	Ubiquitination	Uniprot
K57	Acetylation	Uniprot
K57	Ubiquitination	Uniprot
Y86	Phosphorylation	Uniprot

Research Backgrounds

P60903_S10AA_HUMAN

Function:

Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.

Subunit Structure:

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with SCN10A (By similarity).

Family&Domains:

Belongs to the S-100 family.

References

1). Melatonin attenuates reactive astrogliosis and glial scar formation following cerebral ischemia and reperfusion injury mediated by GSK‐3β and RIP1K. JOURNAL OF CELLULAR PHYSIOLOGY, 2022 (PubMed: 34825376) [IF=5.6]

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S100A10 Antibody - #DF6738