PSMC2 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from MCF7, using PSMC2 Antibody.

DF6736 at 1/100 staining Rat lung tissue by IHC-P.

Product:	PSMC2 Antibody
Catalog:	DF6736
Description:	Rabbit polyclonal antibody to PSMC2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	48kDa; 49kD(Calculated).
Uniprot:	P35998
RRID:	AB_2838698

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

PSMC2 Antibody detects endogenous levels of total PSMC2.

RRID:

AB_2838698
Cite Format: Affinity Biosciences Cat# DF6736, RRID:AB_2838698.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

26S protease complex; 26S protease regulatory subunit 7; 26S proteasome AAA-ATPase subunit RPT1; mammalian suppressor of sgv-1 of yeast; MSS1; Nbla10058; protease 26S subunit 7; proteasome 26S ATPase subunit 2; Proteasome 26S subunit ATPase 2; Proteasome 26S subunit ATPase 2; Protein MSS1; PRS7_HUMAN; PSMC2; putative protein product of Nbla10058; S7;

Immunogens

Immunogen:

Uniprot:

P35998(PRS7_HUMAN) >>Visit HPA database.

Gene(ID):

PSMC2(5701)

Description:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Sequence:

P35998 PRS7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPDYLGADQRKTKEDEKDDKPIRALDEGDIALLKTYGQSTYSRQIKQVEDDIQQLLKKINELTGIKESDTGLAPPALWDLAADKQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSYAKFSATPRYMTYN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35998 As Substrate

P35998

Site	PTM Type	Source
Y4	Phosphorylation	Uniprot
K11	Ubiquitination	Uniprot
K13	Ubiquitination	Uniprot
K17	Ubiquitination	Uniprot
K20	Ubiquitination	Uniprot
K34	Acetylation	Uniprot
K34	Ubiquitination	Uniprot
K46	Ubiquitination	Uniprot
K57	Ubiquitination	Uniprot
K58	Ubiquitination	Uniprot
T63	Phosphorylation	Uniprot
K66	Ubiquitination	Uniprot
K84	Ubiquitination	Uniprot
K100	Ubiquitination	Uniprot
K110	Ubiquitination	Uniprot
Y111	Phosphorylation	Uniprot
K116	Acetylation	Uniprot
K116	Ubiquitination	Uniprot
K120	Ubiquitination	Uniprot
T132	Phosphorylation	Uniprot
K146	Ubiquitination	Uniprot
K156	Ubiquitination	Uniprot
K169	Ubiquitination	Uniprot
Y174	Phosphorylation	Uniprot
K181	Ubiquitination	Uniprot
K186	Ubiquitination	Uniprot
T193	Phosphorylation	Uniprot
K210	Ubiquitination	Uniprot
K222	Ubiquitination	Uniprot
C236	S-Nitrosylation	Uniprot
S243	Phosphorylation	Uniprot
K248	Acetylation	Uniprot
K248	Ubiquitination	Uniprot
Y249	Phosphorylation	Uniprot
K267	Ubiquitination	Uniprot
K268	Ubiquitination	Uniprot
C270	S-Nitrosylation	Uniprot
K316	Sumoylation	Uniprot
K316	Ubiquitination	Uniprot
K340	Ubiquitination	Uniprot
K356	Ubiquitination	Uniprot
C389	S-Nitrosylation	Uniprot
K402	Ubiquitination	Uniprot
T405	Phosphorylation	Uniprot
K407	Acetylation	Uniprot
K407	Ubiquitination	Uniprot
K415	Ubiquitination	Uniprot
K418	Ubiquitination	Uniprot
K422	Acetylation	Uniprot
K422	Ubiquitination	Uniprot
T426	Phosphorylation	Uniprot
R428	Methylation	Uniprot
Y429	Phosphorylation	Uniprot
Y432	Phosphorylation	Uniprot

Research Backgrounds

P35998_PRS7_HUMAN

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC2 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Subcellular Location:

Cytoplasm.
Note: Colocalizes with TRIM5 in cytoplasmic bodies.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC2 and few additional components. Interacts with NDC80/HEC; this interaction is detected only during M phase. Interacts and SQSTM1. Interacts with PAAF1. Directly interacts with TRIM5.

Family&Domains:

Belongs to the AAA ATPase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PSMC2 Antibody - #DF6736