HSD17B2 Antibody - #DF6734

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Product: HSD17B2 Antibody
Catalog: DF6734
Description: Rabbit polyclonal antibody to HSD17B2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Dog
Mol.Wt.: 42kDa; 43kD(Calculated).
Uniprot: P37059
RRID: AB_2838696

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Dog(100%)
Clonality:
Polyclonal
Specificity:
HSD17B2 Antibody detects endogenous levels of total HSD17B2.
RRID:
AB_2838696
Cite Format: Affinity Biosciences Cat# DF6734, RRID:AB_2838696.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

17 beta HSD 2; 17-beta-HSD 2; 17-beta-hydroxysteroid dehydrogenase type 2; 20 alpha HSD; 20 alpha hydroxysteroid dehydrogenase; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; DHB2_HUMAN; E2DH; EDH17B2; Estradiol 17-beta-dehydrogenase 2; HSD17; Hsd17b2; Hydroxysteroid (17 beta) dehydrogenase 2; Microsomal 17 beta hydroxysteroid dehydrogenase; Microsomal 17-beta-hydroxysteroid dehydrogenase; SDR9C2; Short chain dehydrogenase/reductase family 9C, member 2; Testosterone 17 beta dehydrogenase; Testosterone 17-beta-dehydrogenase;

Immunogens

Immunogen:
Uniprot:

P37059(DHB2_HUMAN) >>Visit HPA database.

Gene(ID):
HSD17B2(3294)
Sequence:
MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLLILSPFWGLILFSVSCFLMYTYLSGQELLPVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICLAHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Xenopus
60
Pig
0
Horse
0
Bovine
0
Sheep
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P37059 As Substrate

Site PTM Type Enzyme
K141 Acetylation
S251 Phosphorylation
S259 Phosphorylation
T267 Phosphorylation
S273 Phosphorylation

Research Backgrounds

Function:

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

Subcellular Location:

Membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

References

1). Single-cell RNA sequencing reveals that HSD17B2 in cancer-associated fibroblasts promotes the development and progression of castration-resistant prostate cancer. Cancer Letters, 2023 (PubMed: 37244445) [IF=9.7]
2). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology, 2020 (PubMed: 32457626) [IF=5.6]

Application: WB    Species: Human    Sample: LC540 (17β-HSD3) cells

Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17β-HSD3 mRNA. (D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 μM, while the concentration of 3858858 (positive control) was 0.25 μM. (E) T produced from the microsomal fraction of rat testes containing 17β-HSD3. The data are reported as the mean ± sd (n = 3) of results obtained from individual experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 are considered to be significant, vs. the control.
3). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology, 2023 (PubMed: 36731722) [IF=5.0]

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