RPL5 Antibody - #DF6730

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Product: RPL5 Antibody
Catalog: DF6730
Description: Rabbit polyclonal antibody to RPL5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 34kDa; 34kD(Calculated).
Uniprot: P46777
RRID: AB_2838692

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL5 Antibody detects endogenous levels of total RPL5.
RRID:
AB_2838692
Cite Format: Affinity Biosciences Cat# DF6730, RRID:AB_2838692.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L5; DBA6; L5; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; Ribosomal protein L5; RL5; RL5_HUMAN; RP L5; RPL 5; RPL5; U21 RNA; U21RNA;

Immunogens

Immunogen:
Uniprot:

P46777(RL5_HUMAN) >>Visit HPA database.

Gene(ID):
RPL5(6125)
Description:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Sequence:
MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Xenopus
100
Chicken
100
Bovine
88
Pig
0
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P46777 As Substrate

Site PTM Type Enzyme
M1 Acetylation
G2 Acetylation
K5 Acetylation
K5 Methylation
K5 Ubiquitination
K19 Ubiquitination
K27 Acetylation
K27 Ubiquitination
Y30 Phosphorylation
Y31 Phosphorylation
K41 Acetylation
K41 Ubiquitination
K43 Acetylation
K43 Ubiquitination
K48 Acetylation
K48 Ubiquitination
Y66 Phosphorylation
C76 S-Nitrosylation
C100 S-Nitrosylation
K158 Ubiquitination
K164 Methylation
K164 Sumoylation
K164 Ubiquitination
S172 Phosphorylation
S176 Phosphorylation
K178 Acetylation
K178 Ubiquitination
Y183 Phosphorylation
S185 Phosphorylation
S187 Phosphorylation
K188 Sumoylation
K188 Ubiquitination
K197 Acetylation
K197 Sumoylation
K197 Ubiquitination
Y207 Phosphorylation
R209 Methylation
Y219 Phosphorylation
K220 Acetylation
K220 Sumoylation
K220 Ubiquitination
K221 Acetylation
K221 Sumoylation
K221 Ubiquitination
K228 Methylation
K228 Ubiquitination
S230 Phosphorylation
T232 Phosphorylation
Y240 Phosphorylation
K241 Acetylation
K242 Acetylation
K242 Ubiquitination
R248 Methylation
Y253 Phosphorylation
K270 Ubiquitination
S272 Phosphorylation
K276 Acetylation
K283 Methylation
K284 Ubiquitination
S286 Phosphorylation

Research Backgrounds

Function:

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11. Interacts with isoform 1 of NVL in an ATP-dependent manner. Interacts with RRP1B.

Family&Domains:

Belongs to the universal ribosomal protein uL18 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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