LAMP2 Antibody - #DF6719
Product: | LAMP2 Antibody |
Catalog: | DF6719 |
Description: | Rabbit polyclonal antibody to LAMP2 |
Application: | WB IHC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
Mol.Wt.: | 45kDa,110kDa(Glycosylated); 45kD(Calculated). |
Uniprot: | P13473 |
RRID: | AB_2838681 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF6719, RRID:AB_2838681.
Fold/Unfold
CD_antigen=CD107b;CD107 antigen like family member B;CD107 antigen-like family member B;CD107b;LAMP 2;Lamp 2b;LAMP-2;LAMP2;LAMP2_HUMAN;LAMPB;LGP110;Lysosomal associated membrane protein 2;Lysosome associated membrane glycoprotein 2;Lysosome-associated membrane glycoprotein 2;Lysosome-associated membrane protein 2;MAC3;
Immunogens
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle (PubMed:7488019, PubMed:26856698). Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver (PubMed:7488019, PubMed:26856698). Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta (PubMed:26856698).
- P13473 LAMP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVCFRLFPVPGSGLVLVCLVLGAVRSYALELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNFLVPIAVGAALAGVLILVLLAYFIGLKHHHAGYEQF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P13473 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
N32 | N-Glycosylation | Uniprot | |
N38 | N-Glycosylation | Uniprot | |
N49 | N-Glycosylation | Uniprot | |
N58 | N-Glycosylation | Uniprot | |
T63 | Phosphorylation | Uniprot | |
T65 | Phosphorylation | Uniprot | |
S67 | Phosphorylation | Uniprot | |
N75 | N-Glycosylation | Uniprot | |
N101 | N-Glycosylation | Uniprot | |
N123 | N-Glycosylation | Uniprot | |
N179 | N-Glycosylation | Uniprot | |
S195 | O-Glycosylation | Uniprot | |
T196 | O-Glycosylation | Uniprot | |
T200 | O-Glycosylation | Uniprot | |
T203 | O-Glycosylation | Uniprot | |
T204 | O-Glycosylation | Uniprot | |
S207 | O-Glycosylation | Uniprot | |
T209 | O-Glycosylation | Uniprot | |
T210 | O-Glycosylation | Uniprot | |
T211 | O-Glycosylation | Uniprot | |
T211 | Phosphorylation | Uniprot | |
T213 | O-Glycosylation | Uniprot | |
T213 | Phosphorylation | Uniprot | |
N229 | N-Glycosylation | Uniprot | |
N242 | N-Glycosylation | Uniprot | |
N257 | N-Glycosylation | Uniprot | |
S267 | Phosphorylation | Uniprot | |
T269 | Phosphorylation | Uniprot | |
N275 | N-Glycosylation | Uniprot | |
Y281 | Phosphorylation | Uniprot | |
R293 | Methylation | Uniprot | |
N300 | N-Glycosylation | Uniprot | |
Y304 | Phosphorylation | Uniprot | |
N307 | N-Glycosylation | Uniprot | |
S309 | Phosphorylation | Uniprot | |
N317 | N-Glycosylation | Uniprot | |
N356 | N-Glycosylation | Uniprot |
Research Backgrounds
Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation. Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens.
Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
Cell membrane>Single-pass type I membrane protein. Endosome membrane>Single-pass type I membrane protein. Lysosome membrane>Single-pass type I membrane protein. Cytoplasmic vesicle>Autophagosome membrane.
Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta.
Monomer. Homodimer. Homotrimer. Forms large homooligomers. Interacts (via its cytoplasmic region) with HSPA8. Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (By similarity). Interacts with MLLT11.
Belongs to the LAMP family.
Research Fields
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
References
Application: IHC Species: Mouse Sample:
Application: WB Species: Rat Sample:
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