MCM6 Antibody - #DF6716

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Product: MCM6 Antibody
Catalog: DF6716
Description: Rabbit polyclonal antibody to MCM6
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit
Mol.Wt.: 93kDa, 120 kDa; 93kD(Calculated).
Uniprot: Q14566
RRID: AB_2838678

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(86%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
MCM6 Antibody detects endogenous levels of total MCM6.
RRID:
AB_2838678
Cite Format: Affinity Biosciences Cat# DF6716, RRID:AB_2838678.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA replication licensing factor MCM6; MCG40308; Mcm6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); MCM6 minichromosome maintenance deficient 6; MCM6_HUMAN; Minichromosome maintenance complex component 6; Minichromosome maintenance deficient (mis5 S. pombe) 6; Minichromosome maintenance deficient 6 homolog; Minichromosome maintenance protein 6; Mis5; MIS5 homolog; p105MCM;

Immunogens

Immunogen:
Uniprot:

Q14566(MCM6_HUMAN) >>Visit HPA database.

Gene(ID):
MCM6(4175)
Description:
The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027].
Sequence:
MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNTLVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTRHKIRELTSSRIGLLTRISGQVVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNICRNPVCANRRRFLLDTNKSRFVDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGDKCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLACCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTSGKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Rabbit
100
Sheep
86
Bovine
71
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14566 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S13 Phosphorylation
K28 Ubiquitination
K95 Ubiquitination
K102 Ubiquitination
K108 Ubiquitination
Y111 Phosphorylation
T119 Phosphorylation
T127 Phosphorylation
S153 Phosphorylation
K173 Ubiquitination
T195 Phosphorylation
K197 Ubiquitination
K205 Methylation
K205 Ubiquitination
R217 Methylation
S219 Phosphorylation
K241 Ubiquitination
K256 Ubiquitination
S258 Phosphorylation
T259 Phosphorylation
T266 Phosphorylation
S271 Phosphorylation
Y276 Phosphorylation
T278 Phosphorylation
R282 Methylation
Y294 Phosphorylation
K313 Acetylation
K313 Ubiquitination
R316 Methylation
S324 Phosphorylation
K326 Ubiquitination
K332 Ubiquitination
K336 Ubiquitination
K344 Acetylation
K344 Ubiquitination
K365 Ubiquitination
T380 Phosphorylation
S385 Phosphorylation
K402 Ubiquitination
K407 Ubiquitination
S413 Phosphorylation
K422 Ubiquitination
S424 Phosphorylation
S425 Phosphorylation
K486 Ubiquitination
K490 Ubiquitination
S507 Phosphorylation
K517 Acetylation
K517 Ubiquitination
Y546 Phosphorylation
S557 Phosphorylation
R565 Methylation
S568 Phosphorylation
R573 Methylation
K588 Ubiquitination
S590 Phosphorylation
K599 Acetylation
K599 Ubiquitination
K611 Ubiquitination
T617 Phosphorylation
K643 Acetylation
K643 Ubiquitination
K646 Acetylation
K646 Ubiquitination
K654 Ubiquitination
T661 Phosphorylation
S689 Phosphorylation
S718 Phosphorylation
Y720 Phosphorylation
K733 Ubiquitination
K744 Ubiquitination
K754 Ubiquitination
S758 Phosphorylation
S762 Phosphorylation
K769 Ubiquitination
K775 Acetylation
K775 Ubiquitination
T791 Phosphorylation
K796 Ubiquitination
Y810 Phosphorylation

Research Backgrounds

Function:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

PTMs:

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular Location:

Nucleus.
Note: Binds to chromatin during G1 and detach from it during S phase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). May interact with MCM10. Interacts with TIPIN. Interacts with CDT1. Interacts with MCMBP. Interacts with DDI2.

Family&Domains:

Belongs to the MCM family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

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