TCP1 alpha Antibody - #DF6714

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Product: TCP1 alpha Antibody
Catalog: DF6714
Description: Rabbit polyclonal antibody to TCP1 alpha
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 60kDa; 60kD(Calculated).
Uniprot: P17987
RRID: AB_2838676

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TCP1 alpha Antibody detects endogenous levels of total TCP1 alpha.
RRID:
AB_2838676
Cite Format: Affinity Biosciences Cat# DF6714, RRID:AB_2838676.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI528772; c-cpn; CCT alpha; CCT; CCT-alpha; CCT1; Ccta; CCTalpha; D6S230E; MGC133746; p63; T complex 1; T complex protein 1 alpha subunit; T complex protein 1; T-complex homolog TCP1; T-complex protein 1 subunit alpha; T-complex protein 1 subunit alpha B; Tailless complex polypeptide 1; Tailless complex polypeptide 1A; Tailless complex polypeptide 1B; TCP 1 alpha; Tcp-1; TCP-1-alpha; TCP1; TCPA_HUMAN; Tp63; TRic;

Immunogens

Immunogen:
Uniprot:

P17987(TCPA_HUMAN) >>Visit HPA database.

Gene(ID):
TCP1(6950)
Description:
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.
Sequence:
MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSLGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQKILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
70
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17987 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S6 Phosphorylation
R11 Methylation
S12 Phosphorylation
T13 Phosphorylation
S19 Phosphorylation
S27 Phosphorylation
K33 Ubiquitination
K43 Ubiquitination
K63 Ubiquitination
K73 Ubiquitination
C76 S-Nitrosylation
K84 Acetylation
K109 Ubiquitination
K111 Ubiquitination
S119 Phosphorylation
Y121 Phosphorylation
K126 Ubiquitination
C147 S-Nitrosylation
K153 Ubiquitination
S157 Phosphorylation
S158 Phosphorylation
Y181 Phosphorylation
T182 Phosphorylation
R185 Methylation
S194 Phosphorylation
K199 Acetylation
K199 Methylation
K199 Ubiquitination
S208 Phosphorylation
K233 Ubiquitination
C236 S-Nitrosylation
S240 Phosphorylation
K243 Ubiquitination
K247 Ubiquitination
T255 Phosphorylation
K259 Acetylation
K259 Ubiquitination
Y299 Phosphorylation
C357 S-Nitrosylation
K365 Acetylation
K365 Ubiquitination
K368 Ubiquitination
C397 S-Nitrosylation
K400 Acetylation
K400 Ubiquitination
S429 Phosphorylation
R443 Methylation
S444 Phosphorylation
S460 Phosphorylation
K466 Ubiquitination
R468 Methylation
K481 Ubiquitination
K484 Acetylation
K484 Ubiquitination
S491 Phosphorylation
K494 Ubiquitination
K499 Ubiquitination
K510 Ubiquitination
S513 Phosphorylation
K515 Ubiquitination
K532 Acetylation
K532 Ubiquitination
S537 Phosphorylation
K538 Acetylation
K538 Ubiquitination
K541 Acetylation
K541 Ubiquitination
S544 Phosphorylation
Y545 Phosphorylation
S551 Phosphorylation

Research Backgrounds

Function:

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Interacts with GBA.

Family&Domains:

Belongs to the TCP-1 chaperonin family.

References

1). Histone deacetylase inhibitor induced pVHL-independent degradation of HIF-1α and hierarchical quality control of pVHL via chaperone system. PLoS One, 2021 (PubMed: 34329303) [IF=3.7]

Application: WB    Species: Human    Sample: OV-90 and SKOV-3 cell

Fig 8 TCP-1α expression after LBH589 treatment for 48 h. A) LBH589 suppresses expression of TCP-1α in OV-90 and SKOV-3 cell lines. Data are shown as mean ± SD of at least three individual experiments. (*P < 0.05, **P < 0.01, ***P < 0.001 vs. SKOV-3 control (0.1% DMSO); #P < 0.05, ##P < 0.01, ###P < 0.001 vs. OV-90 control (0.1% DMSO)). B) The expression of TCP-1α was assessed by western blot analysis after LBH589 treatment for 48 h. The equal protein loading is shown by detection of β-actin. The figures show representative blots which were cropped from original images.

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