PSMD10 Antibody - #DF6713
| Product: | PSMD10 Antibody |
| Catalog: | DF6713 |
| Description: | Rabbit polyclonal antibody to PSMD10 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Zebrafish, Horse, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 24kDa; 24kD(Calculated). |
| Uniprot: | O75832 |
| RRID: | AB_2838675 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF6713, RRID:AB_2838675.
Fold/Unfold
26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; Ankyrin repeat protein; dJ889N15.2; Gankyrin; Hepatocellular carcinoma-associated protein p28 II; p28; p28(GANK); Proteasome (prosome, macropain) 26S subunit, non ATPase, 10; PSD10_HUMAN; Psmd10;
Immunogens
Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level).
- O75832 PSD10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGCVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75832 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K18 | Ubiquitination | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| Y138 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K221 | Acetylation | Uniprot | |
| K221 | Ubiquitination | Uniprot |
Research Backgrounds
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.
Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.
Cytoplasm. Nucleus.
Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level).
Part of transient complex containing PSMD10, PSMC4, PSMC5 and PAAF1 formed during the assembly of the 26S proteasome. Stays associated throughout the assembly of the PA700/19S RC and is released upon association with the 20S core. Interacts with PSMC4. Interacts with RB1. Interacts with CDK4. Interacts with MDM2. Interacts with RELA. Associates with a CDK4:CCND2 serine/threonine kinase complex. Interacts with ARHGDIA and increases the interaction between ARHGDIA and RHOA, hence promotes ARHGDIA inactivation of RHOA and ROCK.
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