ODC1 Antibody | Affinity Biosciences

WB
IHC
Cites

1/8

Western blot analysis of extracts from 3T3-L1P6(LPS 4h treatment), using ODC1 Antibody.

DF6712 at 1/100 staining Mouse heart tissue by IHC-P.

DF6712 at 1/100 staining Mouse testis tissue by IHC-P.

DF6712 at 1/100 staining Rat testis tissue by IHC-P.

FIGURE 3|IL-33 induces macrophage ornithine decarboxylase (ODC) activation.

Fig.

Figure 5 The effects of different concentrations of UA alone or in combination with Dox on ODC and phosphorylation of Erk1/2, VEGF, MMP-9.

Product:	ODC1 Antibody
Catalog:	DF6712
Description:	Rabbit polyclonal antibody to ODC1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	51kDa; 51kD(Calculated).
Uniprot:	P11926
RRID:	AB_2838674

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Zebrafish(83%), Bovine(92%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

ODC1 Antibody detects endogenous levels of total ODC1.

RRID:

AB_2838674
Cite Format: Affinity Biosciences Cat# DF6712, RRID:AB_2838674.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DCOR_HUMAN; Dodc1; Odc 1; ODC; Odc1; ODC2; Ornithine decarboxylase 1; Ornithine decarboxylase 2; Ornithine decarboxylase; Ornithine decarboxylase structural 1; RNODC;

Immunogens

Immunogen:

Uniprot:

P11926(DCOR_HUMAN) >>Visit HPA database.

Gene(ID):

ODC1(4953)

Description:

Ornithine decarboxylase (ODC) is also named as ODC1and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control(PMID:8486633). This protein can be phosphorylated in vivo(PMID:8798774). This antibody is specific to ODC1.

Sequence:

P11926 DCOR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Sheep

100

Dog

100

Chicken

100

Bovine

Xenopus

Rabbit

Pig

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11926 As Substrate

P11926

Site	PTM Type	Source
K27	Ubiquitination	Uniprot
K37	Ubiquitination	Uniprot
K50	Ubiquitination	Uniprot
K57	Ubiquitination	Uniprot
K69	Ubiquitination	Uniprot
K78	Ubiquitination	Uniprot
K115	Ubiquitination	Uniprot
K141	Ubiquitination	Uniprot
K150	Ubiquitination	Uniprot
K161	Ubiquitination	Uniprot
K247	Ubiquitination	Uniprot
K261	Ubiquitination	Uniprot
S303	Phosphorylation	Uniprot
C360	S-Nitrosylation	Uniprot

Research Backgrounds

P11926_DCOR_HUMAN

Function:

Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.

PTMs:

S-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues.

Subunit Structure:

Homodimer. Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (Probable). Does not form a heterodimer with AZIN2 (By similarity).

Family&Domains:

Belongs to the Orn/Lys/Arg decarboxylase class-II family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Dissecting the single-cell transcriptome network underlying esophagus non-malignant tissues and esophageal squamous cell carcinoma. eBioMedicine, 2021 (PubMed: 34192657) [IF=11.1]

Application: IF/ICC Species: Human Sample: ESCC and non-malignant tissues

Fig. 3 The single-cell transcriptomes of epithelial cells in non-malignant and malignant esophagus. (a) The TSNE plot and overview of epithelial cells by the cell type and origin of the cells. (b) Canonical cell markers were used to label epithelial subtypes as represented in the TSNE plot. (c) The TSNE plot of epithelial cells by the CNV's levels of the cells. (d) Heatmap showing the expression of marker genes in malignant and non-malignant epithelial cells. (e) Violin plots showing the marker genes for each epithelial cell type. (f) Immunofluorescence staining of tumor (n = 8) (up) / normal (n = 8) (down) tissue-specific genes in ESCC and non-malignant tissues. Comparison of non-malignant and malignant esophagus was performed by Wilcoxon test. (g) Pseudotime analysis for malignant and non-malignant epithelial cells. (h) Differences in pathway activities scored per cell by GSVA between malignant and non-malignant epithelial cells. The scores of pathways are normalized.

2). Oesophageal squamous cell carcinoma–associated IL‐33 rewires macrophage polarization towards M2 via activating ornithine decarboxylase. CELL PROLIFERATION, 2021 (PubMed: 33305406) [IF=8.5]

Application: WB Species: human Sample: M2 macrophages

FIGURE 3|IL-33 induces macrophage ornithine decarboxylase (ODC) activation. A, ODC and arginase 1 (ARG1) protein levels were determined by Western blot, and GAPDH was used as a reference control.

Application: IF/ICC Species: human Sample: M2 macrophages

FIGURE 3|IL-33 induces macrophage ornithine decarboxylase (ODC) activation. A, ODC and arginase 1 (ARG1) protein levels were determined by Western blot, and GAPDH was used as a reference control. B, Relative intensity of ODC and ARG1 protein. C, Immunofluorescence staining for IL-33–induced M2 macrophages. The green signal represents the staining of activated ODC, and the blue signal represents the DAPI-stained nuclei (scale bar = 20 μm). *P < .05; **P < .01; ***P < .001. Bars indicate mean and SEM of triplicate experiments and show a representative experiment of at least 3 independent experiments performed for each panel

3). Inhibitory Effect of Ursolic Acid on the Migration and Invasion of Doxorubicin-Resistant Breast Cancer. MOLECULES, 2022 (PubMed: 35209071) [IF=4.6]

Application: WB Species: Human Sample: MCF-7/ADR Cells

Figure 5 The effects of different concentrations of UA alone or in combination with Dox on ODC and phosphorylation of Erk1/2, VEGF, MMP-9. (A): representative immunoreactive bands of ODC, VEGF, MMP-9, GAPDH, total and phosphorylated Erk1/2 using specific antibody. (B–E): representative quantification of ODC, phosphorylated Erk1/2, VEGF, MMP-9. (F): determination of MMP-9 activity. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with MCF-7/ADR cells, ## p < 0.01, ### p < 0.001, comparison between MCF-7/ADR group and MCF-7 group.

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ODC1 Antibody - #DF6712