GPC3 Antibody - #DF6710

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Product: GPC3 Antibody
Catalog: DF6710
Description: Rabbit polyclonal antibody to GPC3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 66kDa; 66kD(Calculated).
Uniprot: P51654
RRID: AB_2838672

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(90%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
GPC3 Antibody detects endogenous levels of total GPC3.
RRID:
AB_2838672
Cite Format: Affinity Biosciences Cat# DF6710, RRID:AB_2838672.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DGSX; Glypican proteoglycan 3; Glypican-3 [Precursor]; Gpc3; GPC3_HUMAN; GTR2 2; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI 5; Intestinal protein OCI-5; MXR7; OCI 5; OCI-5; OCI5; SDYS; Secreted glypican-3; SGB; SGBS; SGBS1;

Immunogens

Immunogen:
Uniprot:

P51654(GPC3_HUMAN) >>Visit HPA database.

Gene(ID):
GPC3(2719)
Expression:
P51654 GPC3_HUMAN:

Highly expressed in lung, liver and kidney.

Description:
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
Sequence:
MAGTVRTACLVVAMLLSLDFPGQAQPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHSPLKLLTSMAISVVCFFFLVH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Zebrafish
90
Chicken
89
Xenopus
67
Pig
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51654 As Substrate

Site PTM Type Enzyme
K50 Ubiquitination
N124 N-Glycosylation
N241 N-Glycosylation
Y299 Phosphorylation
Y311 Phosphorylation
S352 Phosphorylation
S359 O-Glycosylation
N418 N-Glycosylation
K442 Ubiquitination
S571 Phosphorylation

Research Backgrounds

Function:

Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).

PTMs:

O-glycosylated; contains heparan sulfate.

Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor. This processing is essential for its role in inhibition of hedgehog signaling. A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface.

Subcellular Location:

Cell membrane>Lipid-anchor>Extracellular side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in lung, liver and kidney.

Subunit Structure:

Heterodimer; disulfide-linked. Cleavage by a furin-like convertase results in production of alpha and beta chains which form a disulfide-linked heterodimer. Interacts with DPP4. Interacts with FGF2 (By similarity). Interacts with WNT5A. Also interacts with WNT3A and WNT7B. Interacts with hedgehog protein SHH; the heparan sulfate chains are not required for the interaction (By similarity). Also interacts with hedgehog protein IHH (By similarity). Interacts with CD81 (By similarity). Interacts with Wnt receptors FZD4, FZD7 and FZD8; the heparan sulfate chains are required for the interaction.

Family&Domains:

Belongs to the glypican family.

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). Reduced expression of CENP-E contributes to the development of hepatocellular carcinoma and is associated with adverse clinical features. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 31881483) [IF=7.5]

Application: IHC    Species: mouse    Sample: tumor

Fig. 5.| Down regulation of CENP-E in HCC cells promoted tumor growth of in vivo.(C) Tumor sections were subjected to IHC staining, showing the increased expression of GPC-3 but no difference of ARG-1 expression in CENP-E-low-expressing tumors versus control tumors (ARG-1:P > 0.05, GPC-3:P < 0.05). 40× magnifications, scale bar 100 μm. Statistical significance was tested by Student’s t-test.
2). Enhancing anti-tumor efficacy and immune memory by combining 3p-GPC-3 siRNA treatment with PD-1 blockade in hepatocellular carcinoma. OncoImmunology, 2022 (PubMed: 36524206) [IF=7.2]
3). Piezo1 impairs hepatocellular tumor growth via deregulation of the MAPK-mediated YAP signaling pathway. Cell Calcium, 2021 (PubMed: 33610907) [IF=4.0]
4). Production and characterization of GPC3-N protein and its nanobody. PROTEIN EXPRESSION AND PURIFICATION, 2022 (PubMed: 35436616) [IF=1.6]

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