U2AF2 Antibody - #DF6704
| Product: | U2AF2 Antibody |
| Catalog: | DF6704 |
| Description: | Rabbit polyclonal antibody to U2AF2 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog, Xenopus |
| Mol.Wt.: | 53kDa; 54kD(Calculated). |
| Uniprot: | P26368 |
| RRID: | AB_2838666 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF6704, RRID:AB_2838666.
Fold/Unfold
hU2AF(65); hU2AF65; Splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 small nuclear RNA auxiliary factor 2; U2 snRNP auxiliary factor large subunit; U2af2; U2AF2_HUMAN; U2AF65;
Immunogens
- P26368 U2AF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P26368 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| R67 | Methylation | Uniprot | |
| K70 | Acetylation | Uniprot | |
| K70 | Sumoylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T124 | Phosphorylation | Uniprot | |
| T126 | Phosphorylation | Uniprot | |
| T133 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| K195 | Methylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| T437 | Phosphorylation | Uniprot | |
| R457 | Methylation | Uniprot | |
| K462 | Acetylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| C464 | S-Nitrosylation | Uniprot |
Research Backgrounds
Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals.
Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.
Nucleus.
Interacts with U2AF1L4 (By similarity). Heterodimer with U2AF1. Binds unphosphorylated SF1. Interacts with SCAF11 and SNW1. Interacts with ZRSR2/U2AF1-RS2. Interacts with RBM17. Interacts with PRPF19; the interaction is direct. Interacts with POLR2A (via the C-terminal domain); recruits PRPF19 and the Prp19 complex to the pre-mRNA. Interacts with KHDC4 (Isoform 2). Interacts with ZRSR2. Interacts with the SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A. Interacts (via N-terminus) with CPSF7 (via C-terminus); this interaction stimulates pre-mRNA 3'-end processing by promoting the recruitment of the CFIm complex to cleavage and polyadenylation signals.
Belongs to the splicing factor SR family.
Research Fields
· Genetic Information Processing > Transcription > Spliceosome.
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