EBAG9 Antibody - #DF6703

(4)  
Product: EBAG9 Antibody
Catalog: DF6703
Description: Rabbit polyclonal antibody to EBAG9
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 24kDa; 24kD(Calculated).
Uniprot: O00559
RRID: AB_2838665

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
EBAG9 Antibody detects endogenous levels of total EBAG9.
RRID:
AB_2838665
Cite Format: Affinity Biosciences Cat# DF6703, RRID:AB_2838665.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAG9; Cancer associated surface antigen; Cancer associated surface antigen RCAS1; Cancer-associated surface antigen RCAS1; EB9; EBAG 9; EBAG9; Estrogen receptor binding fragment associated gene 9 protein; Estrogen receptor binding site associated antigen 9; estrogen receptor binding site associated, antigen, 9; Estrogen receptor-binding fragment-associated gene 9 protein; PDAF; RCAS 1; RCAS1; RCAS1_HUMAN; Receptor binding cancer antigen expressed on SiSo cells; Receptor-binding cancer antigen expressed on SiSo cells;

Immunogens

Immunogen:
Uniprot:

O00559(RCAS1_HUMAN) >>Visit HPA database.

Gene(ID):
EBAG9(9166)
Expression:
O00559 RCAS1_HUMAN:

Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.

Description:
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10.
Sequence:
MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Dog
89
Xenopus
78
Zebrafish
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00559 As Substrate

Site PTM Type Enzyme
S36 Phosphorylation
T41 Phosphorylation
Y48 Phosphorylation
S49 Phosphorylation
S50 Phosphorylation
S62 Phosphorylation
T82 Phosphorylation
S86 Phosphorylation
Y94 Phosphorylation
T99 Phosphorylation
T101 Phosphorylation
K112 Ubiquitination
T134 Phosphorylation
K202 Acetylation

Research Backgrounds

Function:

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Subcellular Location:

Golgi apparatus membrane>Single-pass type III membrane protein.
Note: According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.

Subunit Structure:

Homodimer.

Family&Domains:

The coiled coil domain is necessary for the homodimerization.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.